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Transcription profiling of mouse embryonic day (E) 11.5 and E12.5 mouse gonadsNew candidate gene identification for controlling mammalian gonadal sex determination

ABSTRACT: Mammalian gonadal sex determination is dependent on proper expression of sex determining genes in fetal gonadal somatic support cells (i.e., pre-granulosa and pre-Sertoli cells in XX and XY gonads, resp.). We used a unique transgenic mouse strain combined with microarray profiling to identify all the differentially expressed transcripts in XX and XY isolated somatic support cells during critical stages of gonadal development and differentiation. Experiment Overall Design: XX and XY somatic support cells (SSC) were isolated by flow cytometry from embryonic day (E) 11.5 and E12.5 mouse gonads. Total RNA was isolated from pools of isolated cells; 3 pools per sex and each timepoint.

ORGANISM(S): Mus musculus

SUBMITTER: Gerrit Bouma

PROVIDER: E-GEOD-18211 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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New candidate genes identified for controlling mouse gonadal sex determination and the early stages of granulosa and Sertoli cell differentiation.

Bouma Gerrit J GJ Hudson Quanah J QJ Washburn Linda L LL Eicher Eva M EM

Biology of reproduction 20091028 2

Mammalian gonadal sex-determining (GSD) genes are expressed in a unique population of somatic cells that differentiate into granulosa cells in XX gonads or Sertoli cells in XY gonads. The ability to efficiently isolate these somatic support cells (SSCs) during the earliest stages of gonad development would facilitate identifying 1) new candidate GSD genes that may be involved in cases of unexplained abnormal gonad development and 2) genes involved in the earliest stages of granulosa and Sertoli ...[more]

PMID: 19864314

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New candidate gene identification for controlling mammalian gonadal sex determination

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