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Transcription profiling of human A673 Ewing family tumor cell line transfected with interleukin-2 expression vector


ABSTRACT: Response of A673-cells to transfection with interleukin 2 (IL-2). Ewing family tumor (EFT) cell line A673 was transfected with an expression vector for IL-2 (cell line SBVGA1) or empty control vector (cell line SBVGA3). Cells were harvested at 3 different time points and processed for DNA-microarray analysis using Affymetrix HG-U95A microarrays. Primary image analysis was performed using MAS 5.0. In addition, primary analysis was performed with MAS 4.0 and data were scaled to an target intesity of 200. These data have been analysed together with other published data that have been scaled to the same target intensity (see Staege et al. Pediatr Blood Cancer 2004 Jul;43(1):23-34. See also Staege et al. Cancer Res. 2004; in press).

ORGANISM(S): Homo sapiens

SUBMITTER: Martin Sebastian Staege 

PROVIDER: E-GEOD-1823 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Functional and molecular characterization of interleukin-2 transgenic Ewing tumor cells for in vivo immunotherapy.

Staege Martin S MS   Hansen Gesine G   Baersch Gudrun G   Burdach Stefan S  

Pediatric blood & cancer 20040701 1


<h4>Background</h4>Interleukin-2 (IL-2) is a potent cytokine with potential activity against several tumors including Ewing tumors (ET). Side effects of systemic IL-2 can be circumvented by the use of transgenic tumor cells. However, in vitro manipulation may change the overall gene expression profile of tumor cells unfavorably. Therefore, we assessed gene expression profiles, safety, and immunomodulatory efficacy of IL-2 transgenic (IL-2-tg) ET cells in vitro and in NOD/scid mice.<h4>Procedure<  ...[more]

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