Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of Drosophila melanogaster spermatogenesis: mitotic, meiotic and post-meiotic cells


ABSTRACT: We conducted a genome-wide expression analysis of wild-type males using three cell populations isolated from mitotic, meiotic and post-meiotic phases of spermatogenesis in Drosophila melanogaster. Our approach was to directly isolate testis regions enriched with RNAs from each of the three specific germline phases. We used microarrays to detail the global gene expression profile in spermatogenesis and identified up- and down-regulated genes between two different spermatogenic phases in pairwise comparisons Experiment Overall Design: Cell types present at various stages of spermatogenesis are generally located in a gradient along the proximal-distal axis of the testis, however most are not exclusively restricted to any one geographic region. Cells enriched for mitotic, meiotic and post-meiotic phases were obtained by dissection of apical, proximal and distal regions of the testis, respectively

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Maria Vibranovski 

PROVIDER: E-GEOD-18502 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Stage-specific expression profiling of Drosophila spermatogenesis suggests that meiotic sex chromosome inactivation drives genomic relocation of testis-expressed genes.

Vibranovski Maria D MD   Lopes Hedibert F HF   Karr Timothy L TL   Long Manyuan M  

PLoS genetics 20091120 11


In Drosophila, genes expressed in males tend to accumulate on autosomes and are underrepresented on the X chromosome. In particular, genes expressed in testis have been observed to frequently relocate from the X chromosome to the autosomes. The inactivation of X-linked genes during male meiosis (i.e., meiotic sex chromosome inactivation-MSCI) was first proposed to explain male sterility caused by X-autosomal translocation in Drosophila, and more recently it was suggested that MSCI might provide  ...[more]

Similar Datasets

2009-11-24 | GSE18502 | GEO
2010-12-16 | E-GEOD-21447 | biostudies-arrayexpress
2022-08-15 | PXD031779 | Pride
2022-08-15 | PXD031781 | Pride
2019-11-12 | PXD012418 | Pride
2018-11-02 | PXD009220 | Pride
2010-07-21 | E-GEOD-23039 | biostudies-arrayexpress
2010-07-21 | GSE23039 | GEO
2013-06-20 | E-GEOD-43721 | biostudies-arrayexpress
2013-09-29 | E-GEOD-50807 | biostudies-arrayexpress