Male mammalian meiosis and spermiogenesis is critically dependent on the shared functions of the katanin proteins KATNA1 and KATNAL1

ABSTRACT: Katanin microtubule severing enzymes are potent M-phase regulators across eukaryotes. The requirement of katanin enzymatic A-subunits in male mammalian meiosis, however, remains untested. Here, using multiple single and double gene knockout mice, we reveal katanin A-subunits are essential for male meiosis, and in doing so demonstrate functional compensation exists among mammalian katanin A-subunit paralogs. We show KATNA1 and KATNAL1 collectively regulate the male meiotic spindle, germ cell microtubule bulk, in addition to diverse spermatid remodelling events, including Golgi organisation, pro-acrosomal vesicle trafficking and manchette formation. We also define KATNAL1-specific roles in sperm flagella development, manchette regulation, and sperm-epithelial disengagement. Finally, using immunoprecipitation-mass spectrometry approaches we define the KATNA1, KATNAL1, and KATNB1 mammalian testis interactome, which includes a network of cytoskeletal and vesicle trafficking proteins. Collectively, we reveal the presence of multiple katanin A-subunit paralogs in mammalian spermatogenesis allows for both ‘customized cutting’ via neofunctionalization and protective buffering via gene redundancy.

INSTRUMENT(S): Orbitrap Eclipse, Orbitrap Fusion, Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

DISEASE(S): Male Infertility

SUBMITTER: Joel Steele  

LAB HEAD: Jessica Dunleavy

PROVIDER: PXD038404 | Pride | 2023-10-30

REPOSITORIES: Pride