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ModENCODE Drosophila RNA Binding Protein RNAi RNA-Seq Studies

ABSTRACT: Deep Sequencing of mRNA from the Drosophila melanogaster S2-DRSC cells that have been RNAi depleted of mRNAs encoding RNA binding proteins. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Analysis of Poly(A)+ RNA from S2-DRSC Cells depleted of mRNAs encoding RNA binding proteins

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Brenton Graveley

PROVIDER: E-GEOD-18508 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Conservation of an RNA regulatory map between Drosophila and mammals.

Brooks Angela N AN Yang Li L Duff Michael O MO Hansen Kasper D KD Park Jung W JW Dudoit Sandrine S Brenner Steven E SE Graveley Brenton R BR

Genome research 20101004 2

Alternative splicing is generally controlled by proteins that bind directly to regulatory sequence elements and either activate or repress splicing of adjacent splice sites in a target pre-mRNA. Here, we have combined RNAi and mRNA-seq to identify exons that are regulated by Pasilla (PS), the Drosophila melanogaster ortholog of mammalian NOVA1 and NOVA2. We identified 405 splicing events in 323 genes that are significantly affected upon depletion of ps, many of which were annotated as being cons ...[more]

PMID: 20921232

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