Unknown,Transcriptomics,Genomics,Proteomics

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Identification of the Early VIP Transriptome and its Associated Interactome in Activated Murine CD4 T Cells

ABSTRACT: Identification of the Early Vasoactive Intestinal Peptide (VIP) Transriptome and its Associated Interactome in Activated Murine CD4 T Cells In an attempt to understand the biological role of this neuropeptide in the immune system, we choose CD4 T cells as a cellular system for identifying a VIP-induced transcriptome. Murine CD4 T cells were isolated and used to identify changes in gene expression in the presence of PMA/ionomycin (activated) for five hours with and without 10-7 M VIP. Balanced-block design, 6 biological replicates. PMA/ionomycin (activated) mouse CD4 T spleenocytes with VIP ligand (sample) vs. PMA/ionomycin (activated) mouse CD4 T spleenocytes without VIP ligand (control), dye-swaps.

ORGANISM(S): Mus musculus

SUBMITTER: Yulia Dementieva 

PROVIDER: E-GEOD-18525 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of the early VIP-regulated transcriptome and its associated, interactome in resting and activated murine CD4 T cells.

Dorsam Sheri Tinnell ST   Vomhof-Dekrey Emilie E   Hermann Rebecca J RJ   Haring Jodie S JS   Van der Steen Travis T   Wilkerson Erich E   Boskovic Goran G   Denvir James J   Dementieva Yulia Y   Primerano Donald D   Dorsam Glenn Paul GP  

Molecular immunology 20100201 6

More than 40 years after the discovery of vasoactive intestinal peptide (VIP), its transcriptome in the immune system has still not been completely elucidated. In an attempt to understand the biological role of this neuropeptide in immunity, we chose CD4 T cells as a cellular system. Agilent Mouse Whole Genome microarrays were hybridized with fluorescently labeled total RNA isolated from resting CD4 T cells cultured +/-10(-7)M VIP for 5h or PMA/ionomycin activated CD4 T cells cultured +/-10(-7)M  ...[more]

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