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Estrogen Coordinates Translation and Transcription Revealing a Role for NRSF in Human Breast Cancer Cells

ABSTRACT: Analysis of estrogen receptor (ER)-positive MCF7 cell total RNA expression and polysome-assiciated RNA expression following treatment with estradiol (E2) and vehicle (etoh). We used expression microarrays to measure polysome association and total RNA abundance in E2 treated MCF7. These data, along with previously published data, show that genes that are upregulated by estrogen treatment are biased towards association with polysomes. MCF7 cells were grown in hormone depleted media for three days before a 1 hour treatment with E2 or 0.1 % ethanol (vehicle). Total RNA was collected using standard methods and polysome-association RNA from the same cells were collected using sucrose gradient fractionation. Both RNA populations were purified, labeled, and hybridized to Affymetrix Human Genest arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Alexander Brodsky

PROVIDER: E-GEOD-18592 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Estrogen coordinates translation and transcription, revealing a role for NRSF in human breast cancer cells.

Bronson Michael W MW Hillenmeyer Sara S Park Richard W RW Brodsky Alexander S AS

Molecular endocrinology (Baltimore, Md.) 20100414 6

Posttranscriptional regulation may enhance or inhibit estrogen transcriptional control to promote proliferation of breast cancer cells. To understand how transcriptome and translational responses coordinate to drive proliferation, we determined estrogen's global and specific effects on translation regulation by comparing the genome-wide profiles of total mRNA, polysome-associated mRNA, and monosome-associated mRNAs in MCF-7 cells after stimulation by 1 h of 10 nm 17beta-estradiol (E2). We observ ...[more]

PMID: 20392875

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