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Global gene expression analysis of fibroblast, dOB, osteoblast, MSC and MSC-OB

ABSTRACT: Comparison of each cell mRNA expression pattern. We generated osteoblasts like cell by direct reprogramming technique from human fibroblast. To confirm dOB gene expression pattern similarity to osteoblasts, we analyzed and compared the gene expression profiles of the samples by cDNA microarry. These analysis revealed that dOB are similar to osteoblats in comparative level to MSC-OB. 6 samples.

ORGANISM(S): Homo sapiens

SUBMITTER: Kenta Yamamoto

PROVIDER: E-GEOD-52817 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Direct conversion of human fibroblasts into functional osteoblasts by defined factors.

Yamamoto Kenta K Kishida Tsunao T Sato Yoshiki Y Nishioka Keisuke K Ejima Akika A Fujiwara Hiroyoshi H Kubo Toshikazu T Yamamoto Toshiro T Kanamura Narisato N Mazda Osam O

Proceedings of the National Academy of Sciences of the United States of America 20150427 19

Osteoblasts produce calcified bone matrix and contribute to bone formation and remodeling. In this study, we established a procedure to directly convert human fibroblasts into osteoblasts by transducing some defined factors and culturing in osteogenic medium. Osteoblast-specific transcription factors, Runt-related transcription factor 2 (Runx2), and Osterix, in combination with Octamer-binding transcription factor 3/4 (Oct4) and L-Myc (RXOL) transduction, converted ∼ 80% of the fibroblasts into ...[more]

PMID: 25918395

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