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Genome-Wide Microarray Analysis of Histone H2B Ubiquitylation mutant strains of Tetrahymena thermophila

ABSTRACT: Microarray analyses were performed to compare the gene expression profiles of wild-type and several mutant strains of the ciliated protozoan Tetrahymena thermophila. Elimination of H3K4 methylation (hht2-K4Q) and knockout of either of the ubiquitylation enzymes (delta-UBC2 and delta-BRE1) affects a broader spectrum of genes than elimination of H2B ubiquitylation (htb1-K115R). Cells at mid-logarithmic growing phase (cell density of 200,000 cells/ml) were collected. Then total RNAs were extracted and hybridized.

ORGANISM(S): Tetrahymena thermophila

SUBMITTER: Martin Gorovsky

PROVIDER: E-GEOD-18743 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Histone H2B ubiquitylation is not required for histone H3 methylation at lysine 4 in tetrahymena.

Wang Zhe Z Cui Bowen B Gorovsky Martin A MA

The Journal of biological chemistry 20091011 50

Ubiquitylation of histone H2B and/or a component of the system that ubiquitylates H2B is required for methylation of histone H3 at lysine 4 (H3K4) in yeasts and probably in humans. In this study, the single ubiquitylation site was mapped to conserved lysine 115 of the C-terminal region of histone H2B in the single-cell model organism Tetrahymena thermophila. In strains lacking H2B ubiquitylation, H3K4 methylation was not detectably affected. As in other organisms, the E2 ubiquitin-conjugating en ...[more]

PMID: 19822522

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Genome-Wide Microarray Analysis of Histone H2B Ubiquitylation mutant strains of Tetrahymena thermophila

Project description:Microarray analyses were performed to compare the gene expression profiles of wild-type and several mutant strains of the ciliated protozoan Tetrahymena thermophila. Elimination of H3K4 methylation (hht2-K4Q) and knockout of either of the ubiquitylation enzymes (delta-UBC2 and delta-BRE1) affects a broader spectrum of genes than elimination of H2B ubiquitylation (htb1-K115R).

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