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Cell envelope metabolism in phosphate limited Bacillus subtilis cells

ABSTRACT: Investigation of the whole genome expression level changes in phosphate limited Bacillus subtilis wild-type and delta-phoPR cells Investigation of the whole genome expression level changes of wild-type and delta-phoPR Bacills subtilis cells comparing high and low phosphate medium For each sample analyzed in this study 3 technical replicates were performed. 3 different samples were taken for wild-type cell and delta-phoPR cell, respectively. Samples were taken from exponentially growing cells in high and low phosphate medium as well as from phosphate-limited cells.

ORGANISM(S): Bacillus subtilis

SUBMITTER: Karsten Hokamp

PROVIDER: E-GEOD-23164 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cell envelope gene expression in phosphate-limited Bacillus subtilis cells.

Botella Eric E Hübner Sebastian S Hokamp Karsten K Hansen Annette A Bisicchia Paola P Noone David D Powell Leagh L Salzberg Letal I LI Devine Kevin M KM

Microbiology (Reading, England) 20110602 Pt 9

The high phosphate content of Bacillus subtilis cell walls dictates that cell wall metabolism is an important feature of the PhoPR-mediated phosphate limitation response. Here we report the expression profiles of cell-envelope-associated and PhoPR regulon genes, determined by live cell array and transcriptome analysis, in exponentially growing and phosphate-limited B. subtilis cells. Control by the WalRK two-component system confers a unique expression profile and high level of promoter activity ...[more]

PMID: 21636651

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