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Time course analysis of dedifferentiation in porcine follicular granulosa cells

ABSTRACT: Cellular dedifferentiation signifies the withdrawal of cells from a specific differentiated state into a M-bM-^@M-^Xstem cellM-bM-^@M-^Y-like undifferentiated state. However, the mechanism of dedifferentiation remains obscure. We showed that follicular granulosa cells (GC), which have distinct functions in vivo, can dedifferentiate during culture in vitro and acquire multipotency. We investigated the dedifferentiation of GC using global gene expression analyses. Total RNA was isolated from GCs to DFOG cells at 5 time points during dedifferentiation (cultured in 0day, 1day, 2day, 4day and 7day). Each timepoint was performed in triplicate (ie, biological replicates). Using Affymetrix porcine genome array, we performed microarray time course experiments to analyze gene expression profiles during GC dedifferentiation.

ORGANISM(S): Sus scrofa

SUBMITTER: Hiromasa Ono

PROVIDER: E-GEOD-18854 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dedifferentiated follicular granulosa cells derived from pig ovary can transdifferentiate into osteoblasts.

Oki Yoshinao Y Ono Hiromasa H Motohashi Takeharu T Sugiura Nobuki N Nobusue Hiroyuki H Kano Koichiro K

The Biochemical journal 20121001 2

Transdifferentiation is the conversion of cells from one differentiated cell type into another. How functionally differentiated cells already committed to a specific cell lineage can transdifferentiate into other cell types is a key question in cell biology and regenerative medicine. In the present study we show that porcine ovarian follicular GCs (granulosa cells) can transdifferentiate into osteoblasts in vitro and in vivo. Pure GCs isolated and cultured in Dulbecco's modified Eagle's medium s ...[more]

PMID: 22839299

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Comparative transcriptome analysis of dedifferentiation in porcine mature adipocytes and follicular granulosa cells

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2012-11-21 | GSE18854 | GEO

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Polycomb group gene E(z) is required for spermatogonial dedifferentiation in Drosophila adult testis

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