Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Effects of IGF 1 on CCL-171 fibroblasts


ABSTRACT: To characterize the effects of IGF 1 on CCL171 cells, we cultured pre-starved CCL 171 fibroblasts with or without 50ng/ml IGF 1 for 24 h. We then profiled gene expression changes using Human Exonic Evidence Based Oligonucleotide (HEEBO) microarrays. After stimulation, total RNA was extracted and amplified using a modified Eberwine procedure. The amplified RNA was labeled with the fluorescent dye Cy5 and pooled with Cy3 labeled reference RNA, and then the pooled RNA was hybridized onto HEEBO microarrays. After hybridization and washing, arrays were scanned on a fluorescent microscope scanner. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.

ORGANISM(S): Homo sapiens

SUBMITTER: Michal Rajski 

PROVIDER: E-GEOD-18954 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

IGF-I induced genes in stromal fibroblasts predict the clinical outcome of breast and lung cancer patients.

Rajski Michal M   Zanetti-Dällenbach Rosanna R   Vogel Brigitte B   Herrmann Richard R   Rochlitz Christoph C   Buess Martin M  

BMC medicine 20100105


<h4>Background</h4>Insulin-like growth factor-1 (IGF-I) signalling is important for cancer initiation and progression. Given the emerging evidence for the role of the stroma in these processes, we aimed to characterize the effects of IGF-I on cancer cells and stromal cells separately.<h4>Methods</h4>We used an ex vivo culture model and measured gene expression changes after IGF-I stimulation with cDNA microarrays. In vitro data were correlated with in vivo findings by comparing the results with  ...[more]

Similar Datasets

2010-06-24 | E-GEOD-15361 | biostudies-arrayexpress
2006-01-06 | E-GEOD-3988 | biostudies-arrayexpress
2006-01-27 | E-GEOD-4111 | biostudies-arrayexpress
2010-06-24 | E-GEOD-15297 | biostudies-arrayexpress
2008-02-07 | E-GEOD-7947 | biostudies-arrayexpress
2010-05-12 | E-GEOD-18151 | biostudies-arrayexpress
2007-09-13 | E-GEOD-9027 | biostudies-arrayexpress
2010-06-24 | E-GEOD-16103 | biostudies-arrayexpress
2010-06-24 | E-GEOD-16107 | biostudies-arrayexpress
2005-08-11 | E-GEOD-3107 | biostudies-arrayexpress