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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional profiling of testicular biopsies with Sertoli-cell-only and spermatogonial presence

ABSTRACT: The aim of the study was to identify in vivo spermatogonial gene expression within the context of their biological niche. Identification of spermatogonial genes was done by t-testing on the replicates of Score 3 (Spermatogonia) and Score 2 (SCO) testicular biopsies. 3 biological replicates with spermatogonial presence in the tubuli seminiferi, 5 biological replicates with Sertoli-cell-only syndrome.

ORGANISM(S): Homo sapiens

SUBMITTER: Andrej-Nikolai Spiess

PROVIDER: E-GEOD-18997 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Screening for biomarkers of spermatogonia within the human testis: a whole genome approach.

von Kopylow Kathrein K Kirchhoff Christiane C Jezek Davor D Schulze Wolfgang W Feig Caroline C Primig Michael M Steinkraus Volker V Spiess Andrej-Nikolai AN

Human reproduction (Oxford, England) 20100305 5

<h4>Background</h4>A key step in studying the biology of spermatogonia is to determine their global gene expression profile. However, disassociation of these cells from the testis may alter their profile to a considerable degree. To characterize the molecular phenotype of human spermatogonia, including spermatogonial stem cells (SSCs), within their cognate microenvironment, a rare subtype of human defective spermatogenesis was exploited in which spermatogonia were the only germ cell type.<h4>Met ...[more]

PMID: 20208059

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Transcriptional profiling of testicular biopsies with Sertoli-cell-only and spermatogonial presence

Project description:The aim of the study was to identify in vivo spermatogonial gene expression within the context of their biological niche. Identification of spermatogonial genes was done by t-testing on the replicates of Score 3 (Spermatogonia) and Score 2 (SCO) testicular biopsies.

2009-11-13 | GSE18997 | GEO

Expression data from aged spermatogonial stem cells

Project description:In vitro and in vivo aging of mouse spermatogonial stem cells alters stem cell function based on quantitative spermatogonial stem cell transplantation analyses. We used microarrays to identify differential gene expression in vitro and in vivo aged spermatogonial stem cells to identify potential causes of observed phenotypic differences in aged spermatogonial stem cell function. Spermatogonial stem cells were isolated from young and serial-transplanted aged mouse donors and cultured for short and long periods. Spermatogonial stem cells were isolated from cultures and subjected to microarray analysis to identify differential gene expression.

2011-02-04 | E-GEOD-27043 | biostudies-arrayexpress

Progression of spermatogonial differentiation in the mouse: the pathway to meiosis

Project description:We report bulk RNA-sequencing data for undifferentiated and defined subpopulations of differentiating murine spermatogonia. We have sequenced two replicates each of wild-type and STRA8 knock-out sets of spermatogonia to assess transcriptional changes that occur throughout spermatogonial development prior to meiotic entry.

2020-07-29 | GSE155307 | GEO

Defective germline reprogramming rewires the spermatogonial transcriptome

Project description:Defective germline reprogramming in Miwi2- and Dnmt3l-deficient mice results in the failure to reestablish transposon silencing, meiotic arrest and progressive loss of spermatogonia. Here we sought to understand the molecular basis for this spermatogonial dysfunction. Through a combination of imaging, conditional genetics and transcriptome analysis, we demonstrate that germ cell elimination in the respective mutants arises due to defective germline reprogramming rather than a function for the respective factors within spermatogonia. In both Miwi2-/- and Dnmt3l-/- spermatogonia the intracisternal-A particle (IAP) family of endogenous retroviruses is de-repressed, but in contrast to meiotic cells DNA damage is not observed. Instead we find that unmethylated IAP promoters rewire the spermatogonial transcriptome by driving expression of neighboring host genes. In summary, defective reprogramming deregulates the spermatogonial transcriptome that may underlie spermatogonial dysfunction.

2018-03-09 | E-MTAB-6202 | biostudies-arrayexpress

Expression data from three types of spermatogonial stem cells.

Project description:Multipotent spermatogonial stem cells (mSSCs) derived from SSCs are a potential new source of individualized pluripotent cells in regenerate medicine such as ESCs. We hypothesized that the culture-induced reprogramming of SSCs was mediated by a mechanism different from that of iPS, and was due to up-regulation of specific pluripotency-related genes during cultivation. Through a comparative analysis of expression profile data, we try to find cell reprogramming candidate factors from mouse spermatogonial stem cells. We used microarrays to analyze the gene expression profiles of culture-induced reprogramming converting unipotent spermatogonial stem cells to pluripotent spermatogonial stem cells. Three types of spermatogonial stem cells were mechanically collected according to morphological criteria for RNA extraction and hybridization on Affymetrix microarrays.

2015-10-30 | E-GEOD-74151 | biostudies-arrayexpress

Expression data from aged spermatogonial stem cells

2011-02-04 | GSE27043 | GEO

FGF2-regulated gene expression in cultured mouse spermatogonial stem cells

Project description:Transcriptional profiling of mouse spermatogonial stem cells (SSCs) comparing control untreated SSCs with SSCs with exogenous FGF2 withdrawn and FGFR inhibitor SU5402 supplemented (-F+S). Results provide insight into the mechanisms of FGF2-supported in vitro self-renewal of SSCs. Two-condition experiment, SSCs-F+S vs. SSCs. Biological replicates: 4 control replicates, 4 -F+S replicates.

2010-08-01 | E-GEOD-21294 | biostudies-arrayexpress

Retinoic acid dependent spermatogonial differentiation in mice

Project description:Spermatogonial differentiation is a developmental process that is essential for spermatogenesis, but the molecular and cellular changes that germ cells must undergo to transition from undifferentiated spermatogonia to differentiating spermatogonia remain largely undefined. Retinoic acid (RA) is necessary and sufficient for spermatogonial differentiation. Using the postnatal mouse testis, we examine the transcriptome changes that accompany spermatogonial differentiation. Spermatogenesis was synchronized by administration of potent and selective RA synthesis inhibitor; as a result, testes contained only undifferentiated spermatogonia. Then, the inhibitor was discontinued, and mice were given a single dose of exogenous RA to initiate spermatogonial differentiation. We measured transcriptomes in FACS-enriched germ cells either before RA administration, when the cells correspond to Aal spermatogonia (and a minor contribution of spermatogonial stem cells) or at two points after RA administration, when the cells correspond to A1 or A3 differentiating spermatogonia. The results of this study reveal the full transcriptome changes accompanying spermatogonial differentiation in the mouse.

2023-07-03 | GSE213716 | GEO

Expression data from three types of spermatogonial stem cells.

2015-10-30 | GSE74151 | GEO

Expression data from aging study on mouse spermatogonial stem/progenitor cell population

Project description:In mouse, spermatogonial stem/progenitor cells are the progenitor cell which develop to mature sperms through a series of mitotic and meiotic divisions and differentiation. Gfra1 is an established surface marker for mouse spermatogonial stem/progenitor cells. In this study, we used a transcriptomic approach to investigate the effect of aging on Gfra1-positive and -negative populations of mouse male germ cells. Spermatogonial stem/progenitor cells were isolated from testes of mice at ages 6-day, 21-day, 60-day, and 8-months by magnetic assisted cell sorting (MACS) technique using Gfra1 marker. Transcriptomes among different ages and Gfra1 status were compared.

2015-05-24 | E-GEOD-68298 | biostudies-arrayexpress

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