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Transciptional profile of a M. smegmatis mc2155 ÎsigF strain under standard growth conditions

ABSTRACT: Comparing transciptional profile of a M. smegmatis mc2155 ÎsigF strain and wild type (control) in in vitro culture at two time points (exponential phase and stationary phase) for identification of SigF-dependent genes. Analysis of 2 time points: exponential and stationary phase. 4 biological replicates comparing expression of wild type (control) and ÎsigF strain incl. dye swaps for each time point. 3 technical replicates per array

ORGANISM(S): Mycobacterium smegmatis

SUBMITTER: Susanne Gebhard

PROVIDER: E-GEOD-19145 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The SigF regulon in Mycobacterium smegmatis reveals roles in adaptation to stationary phase, heat, and oxidative stress.

Hümpel Anja A Gebhard Susanne S Cook Gregory M GM Berney Michael M

Journal of bacteriology 20100316 10

SigF is an alternative sigma factor that is highly conserved among species of the genus Mycobacterium. In this study we identified the SigF regulon in Mycobacterium smegmatis using whole-genome microarray and promoter consensus analyses. In total, 64 genes in exponential phase and 124 genes in stationary phase are SigF dependent (P < 0.01, >2-fold expression change). Our experimental data reveal the SigF-dependent promoter consensus GTTT-N((15-17))-GGGTA for M. smegmatis, and we propose 130 pote ...[more]

PMID: 20233930

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