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Unknown,Transcriptomics,Genomics,Proteomics

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Bedaquiline-induced transcriptional response of Mycobacterium smegmatis mc2 155

ABSTRACT: Transcriptional profiling of M. smegmatis mc2155 grown on Hartmans de Bont (HdB) supplemented with 0.2% glycerol and 0.05% Tween80 challenged with 2 μg/ml Bedaquiline and Dimethyl sulfoxide (DMSO) Two-condition experiment. Biological replicates: 5 independently grown and harvested. One replicate per array.

ORGANISM(S): Mycobacterium smegmatis str. MC2 155

SUBMITTER: Jennifer Robson

PROVIDER: E-GEOD-59871 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Bactericidal mode of action of bedaquiline.

Hards Kiel K Robson Jennifer R JR Berney Michael M Shaw Lisa L Bald Dirk D Koul Anil A Andries Koen K Cook Gregory M GM

The Journal of antimicrobial chemotherapy 20150308 7

<h4>Objectives</h4>It is not fully understood why inhibiting ATP synthesis in Mycobacterium species leads to death in non-replicating cells. We investigated the bactericidal mode of action of the anti-tubercular F1Fo-ATP synthase inhibitor bedaquiline (Sirturo™) in order to further understand the lethality of ATP synthase inhibition.<h4>Methods</h4>Mycobacterium smegmatis strains were used for all the experiments. Growth and survival during a bedaquiline challenge were performed in multiple medi ...[more]

PMID: 25754998

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