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Transcription profiling of rat astrocytes in culture and from human optic nerve to identify acutely expressed genes during CNS injury and their expression in cultured astrocytes

ABSTRACT: A robust set of CNS transcript changes was defined by comparing microarray data that describe the injury response of the rat retina [Vazquez-Chona et al., IOVS 2004; GSE1001], brain [Matzilevich et al., J Neurosci Res 2002; GSE1911], and spinal cord [Di Giovanni et al., Ann Neurol 2003; GDS63]. We determined the CNS injury genes that were expressed in cultured astrocytes from rat cortex [GSM34300] and from human optic nerve head [Yang et al., Physiol Genomics 2004; GDS532].

ORGANISM(S): Homo sapiens

SUBMITTER: Eldon Geisert

PROVIDER: E-GEOD-1928 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genetic networks controlling retinal injury.

Vazquez-Chona Felix R FR Khan Amna N AN Chan Chun K CK Moore Anthony N AN Dash Pramod K PK Hernandez M Rosario MR Lu Lu L Chesler Elissa J EJ Manly Kenneth F KF Williams Robert W RW Geisert Eldon E EE

Molecular vision 20051104

<h4>Purpose</h4>The present study defines genomic loci underlying coordinate changes in gene expression following retinal injury.<h4>Methods</h4>A group of acute phase genes expressed in diverse nervous system tissues was defined by combining microarray results from injury studies from rat retina, brain, and spinal cord. Genomic loci regulating the brain expression of acute phase genes were identified using a panel of BXD recombinant inbred (RI) mouse strains. Candidate upstream regulators withi ...[more]

PMID: 16288200

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acute genes during CNS injury and their expression in cultured astrocytes

Project description:A robust set of CNS transcript changes was defined by comparing microarray data that describe the injury response of the rat retina [Vazquez-Chona et al., IOVS 2004; GSE1001], brain [Matzilevich et al., J Neurosci Res 2002; GSE1911], and spinal cord [Di Giovanni et al., Ann Neurol 2003; GDS63]. We determined the CNS injury genes that were expressed in cultured astrocytes from rat cortex [GSM34300] and from human optic nerve head [Yang et al., Physiol Genomics 2004; GDS532]. Keywords: other

2005-07-01 | GSE1928 | GEO

Hippocampal gene expression after brain injury

Project description:RNA samples were prepared from ipsilateral rat hippocampi 3 hr and 24 hr following lateral cortical impact injury and compared to samples from sham-operated controls. For details please see Matzilevich et al., J Neurosci Res 2002 Mar 1;67(5):646-63. Keywords = Brain injury Keywords: time-course

2005-07-01 | GSE1911 | GEO

Transcription profiling of rat ipsilateral rat hippocampus 3 hr and 24 hr following lateral cortical impact injury and compared to samples from sham-operated controls

2008-06-11 | E-GEOD-1911 | biostudies-arrayexpress

Expression data from reactive astrocytes acutely purified from young adult mouse brains

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2012-05-01 | E-GEOD-35338 | biostudies-arrayexpress

Expression data from reactive astrocytes acutely purified from young adult mouse brains

2012-05-02 | GSE35338 | GEO

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Global transcriptomic profiling of ischemic/reperfusion injury in an in vivo Gpx1 -/- transgenic mouse model.

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Single-cell RNA-seq of ASPM mutant ferret embryonic forebrain

Project description:As described in our paper "Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size" (Johnson et al., Nature 2018), we used the standard Drop-seq method and analysis of Macosko et al. (2015) to capture, sequence, and analyze mRNA from single cells from Aspm wild-type, heterozygous, and knock-out littermate ferrets at embryonic day 35. Bulk reference samples were processed using standard Illumina mRNA-seq library prep and sequencing protocols, and the samples were described previously (Johnson, Wang et al., Nature Neurosci 2015)

2018-04-18 | GSE110010 | GEO

Spinal astrocytes RNA-sequencing

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2021-06-16 | GSE158517 | GEO

Comprehensive Microarray Transcriptome Profiling of CD34-Enriched Mouse Keratinocyte Stem Cells

Project description:Recent developments in animal models (Morris et al., 2004; Tumbar et al., 2004) as well as the discovery of cell surface markers (Jones and Watt, 1993; Tani et al., 2000; Trempus et al., 2003; Nijhof et al., 2006) have made it possible to isolate living epidermal hair follicle stem cells (HFSCs) from mouse skin, facilitating the study of the biological and molecular features inherent to HFSCs. A complexity of stem and progenitor cell populations within the hair follicle has been revealed. Here, we report comprehensive profiling of mouse CD34-expressing HFSCs using the Agilent mouse oligo microarray platform in order to extend and enrich the existing HFSC databases. Keywords: gene expression, cell characterization

2008-04-21 | GSE7690 | GEO

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