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Gene expression in circulating mononuclear cells after exposure to ultrafine carbon particles

ABSTRACT: Peripheral blood samples were collected before (0 hour) and at 24 hours after exposure from healthy subjects who participated in previous controlled exposures to ultrafine carbon particles (UFP, 50 microg/m3) or filtered air (FA)(n = 3 each). The exposure time was 2 hours. RNA from mononuclear cell fraction (>85% lymphocytes) was extracted, amplified and hybridized to Affymetrix HU133 plus 2 microarrays. We used microarray to explore significantly altered genes after ultrafine carbon particle exposure. Each subject was exposed to filtered air or ultrafine carbon particles. Two peripheral blood samples (pre- and post-exposure) were taken. Mononuclear cells were isolated for gene expression analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Yuh-Chin Huang

PROVIDER: E-GEOD-19317 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profile in circulating mononuclear cells after exposure to ultrafine carbon particles.

Huang Yuh-Chin T YC Schmitt Michael M Yang Zhonghui Z Que Loretta G LG Stewart Judith C JC Frampton Mark W MW Devlin Robert B RB

Inhalation toxicology 20100801 10

<h4>Context</h4>Exposure to particulate matter (PM) is associated with systemic health effects, but the cellular and molecular mechanisms are unclear.<h4>Objective</h4>We hypothesized that, if circulating mononuclear cells play an important role in mediating systemic effects of PM, they would show gene expression changes following exposure.<h4>Materials and methods</h4>Peripheral blood samples were collected before (0 h) and at 24 h from healthy subjects exposed to filtered air (FA) and ultrafin ...[more]

PMID: 20507211

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