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CCAAT/enhancer-binding protein alpha target genes in myeloid progenitor cells uncovered by chromatin IP

ABSTRACT: CCAAT/enhancer-binding protein alpha (C/EBPM-NM-1) is a lineage-specific transcription factor that directs development of granulocytes. To obtain insights into the genome-wide transcriptome of wild type C/EBPM-NM-1, we have performed chromatin immunoprecipitation on DNA promoter microarrays (ChIP-chip) in the murine 32D myeloid progenitor cell line. These cells expressed an estradiol-inducible form of wild-type C/EBPM-NM-1. In the murine 32D myeloid progenitor cell line, expressing either an estradiol-inducible form of wild-type C/EBPM-NM-1. (C/EBPM-NM-1-ER, n=3, replicates), or as a control ER (n=2, replicates), we have performed chromatin immunoprecipitation on DNA promoter microarrays (ChIP-chip) after 4 hours of estradiol incubation.

ORGANISM(S): Mus musculus

SUBMITTER: Erdogan Taskesen

PROVIDER: E-GEOD-19321 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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HAT: hypergeometric analysis of tiling-arrays with application to promoter-GeneChip data.

Taskesen Erdogan E Beekman Renee R de Ridder Jeroen J Wouters Bas J BJ Peeters Justine K JK Touw Ivo P IP Reinders Marcel J T MJ Delwel Ruud R

BMC bioinformatics 20100521

<h4>Background</h4>Tiling-arrays are applicable to multiple types of biological research questions. Due to its advantages (high sensitivity, resolution, unbiased), the technology is often employed in genome-wide investigations. A major challenge in the analysis of tiling-array data is to define regions-of-interest, i.e., contiguous probes with increased signal intensity (as a result of hybridization of labeled DNA) in a region. Currently, no standard criteria are available to define these region ...[more]

PMID: 20492700

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Mutant-CCAAT/enhancer-binding protein alpha target genes in myeloid progenitor cells by chromatin IP

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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