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Expression data from wildtype Sinorhizobium meliloti Rm1021 and an hfq mutant

ABSTRACT: The RNA-binding protein Hfq is a global regulator, which controls diverse cellular processes in bacteria. To begin understanding the role of Hfq in the Sinorhizobium meliloti-Medicago truncatula nitrogen-fixing symbiosis, we defined free-living and symbiotic phenotypes of an hfq mutant. Over 500 transcripts were differentially accumulated in the hfq mutant of S. meliloti Rm1021 when grown in a shaking culture. Gene expression profiling of Sinorhizobium meliloti Rm1021 or its isogenic hfq deletion mutant, grown to late exponential phase in rich medium, was performed using custom Affymetrix GeneChips.

ORGANISM(S): Sinorhizobium meliloti

SUBMITTER: Melanie Barnett

PROVIDER: E-GEOD-19346 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Role of the Sinorhizobium meliloti global regulator Hfq in gene regulation and symbiosis.

Gao Mengsheng M Barnett Melanie J MJ Long Sharon R SR Teplitski Max M

Molecular plant-microbe interactions : MPMI 20100401 4

The RNA-binding protein Hfq is a global regulator which controls diverse cellular processes in bacteria. To begin understanding the role of Hfq in the Sinorhizobium meliloti-Medicago truncatula nitrogen-fixing symbiosis, we defined free-living and symbiotic phenotypes of an hfq mutant. Over 500 transcripts were differentially accumulated in the hfq mutant of S. meliloti Rm1021 when grown in a shaking culture. Consistent with transcriptome-wide changes, the hfq mutant displayed dramatic alteratio ...[more]

PMID: 20192823

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