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The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (SyrM)

ABSTRACT: We characterized transcriptomes of a strain overexpressing syrM. Our work shows that the syrM transcriptome shares similar gene expression changes to the syrA and nodD3 transcriptomes and that nodD3 and syrA may be the only targets directly activated by SyrM. We propose that most of the gene expression changes observed when nodD3 is overexpressed are due to NodD3 activation of syrM expression, which in turn stimulates SyrM activation of syrA expression. The subsequent increase in SyrA abundance alters activity of the ChvI-ExoS-ExoR circuit, resulting in broad changes in gene expression. Gene expression profiling of a Sinorhizobium meliloti nodD1, nodD2, nodD3 mutant overexpressing syrM was performed using custom Affymetrix GeneChips

ORGANISM(S): Sinorhizobium meliloti

SUBMITTER: Melanie Barnett

PROVIDER: E-GEOD-61525 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (SyrA)

Project description:We characterized transcriptomes of a strain overexpressing syrA. Our work shows that the syrA transcriptome shares similar gene expression changes to the syrM and nodD3 transcriptomes and that nodD3 and syrA may be the only targets directly activated by SyrM. We propose that most of the gene expression changes observed when nodD3 is overexpressed are due to NodD3 activation of syrM expression, which in turn stimulates SyrM activation of syrA expression. The subsequent increase in SyrA abundance alters activity of the ChvI-ExoS-ExoR circuit, resulting in broad changes in gene expression. Gene expression profiling of Sinorhizobium meliloti overexpressing syrA was performed using custom Affymetrix GeneChips

2015-03-31 | E-GEOD-61524 | biostudies-arrayexpress

The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (ChvI-SyrA)

Project description:We characterized transcriptomes of strains containing a K214T mutation in chvI, with and without overexpression of syrA. Our work shows that overexpression of syrA suppresses many of the transcription changes observed in the chvI K214T mutant. Gene expression profiling of a Sinorhizobium meliloti chvI K214 mutant overexpressing syrA was performed using custom Affymetrix GeneChips

2015-03-31 | E-GEOD-61521 | biostudies-arrayexpress

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Project description:Sinorhizobium meliloti lives as a soil saprophyte, and engages in a nitrogen fixing symbiosis with plant roots. To succeed in such diverse environments, the bacteria must continually adjust gene expression. Transcriptional plasticity in eubacteria is often mediated by alternative sigma factors interacting with core RNA polymerase. The S. meliloti genome encodes 14 of these alternative sigmas, including 11 extracytoplasmic function (ECF) sigmas. We used custom Affymetrix Symbiosis Chips to characterize the global transcriptional response of S. meliloti overexpressing the ECF sigma factor, RpoE2. Our work identifies over 200 genes whose expression is dependent on RpoE2. The gene expression profile of Sinorhizobium meliloti strain CL150 carrying the rpoE2 gene on a plasmid, where it was expressed from a melibiose-inducible promoter (the S. meliloti PmelA promoter), was compared to the gene expression profile of CL150 carrying the corresponding empty vector (pCAP11). CL150 is an Rm1021 strain that has been corrected for two point mutations: it is wild type for ecfR1 and pstC.

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Expression data from wildtype Sinorhizobium meliloti Rm1021 and an hfq mutant

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The Conserved Polarity Factor PodJ1 Impacts Multiple Cell Envelope-Associated Functions in Sinorhizobium meliloti

Project description:Although diminutive in size, bacteria possess highly diverse and spatially confined cellular structures. Two related alpha-proteobacteria, Sinorhizobium meliloti and Caulobacter crescentus, serve as models for investigating the genetic basis of morphologic variations. S. meliloti, a symbiont of leguminous plants, synthesizes multiple flagella and no prosthecae, whereas C. crescentus, a freshwater bacterium, has a single polar flagellum and stalk. The podJ gene, originally identified in C. crescentus for its role in polar organelle development, is split into two juxtaposed open reading frames, podJ1 and podJ2, in S. meliloti. Deletion of podJ1 interferes with flagellar motility, exopolysaccharide production, cell envelope integrity, cell division, and normal morphology, but not symbiosis. As in C. crescentus, the S. meliloti PodJ1 protein appears to act as a polarity beacon and localizes to the newer cell pole. Microarray analysis indicates that podJ1 affects the expression of at least 129 genes, the majority of which correspond to observed mutant phenotypes. The combination of phenotypic characterization, microarray analysis, and suppressor identification suggests that PodJ1 controls a core set of conserved elements, including flagellar and pili genes, the signaling proteins PleC and DivK, and the TacA transcriptional activator, while alternate downstream targets have evolved to suit the distinct lifestyles of individual species. Gene expression profiling of Sinorhizobium meliloti Rm1021 or its isogenic podJ1 deletion mutant, grown to mid exponential phase in rich medium, was performed using custom Affymetrix GeneChips.

2012-08-09 | E-GEOD-31325 | biostudies-arrayexpress

The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-03-31 | E-GEOD-61526 | biostudies-arrayexpress

Dual RpoH sigma factors in Sinorhizobium meliloti

Project description:Sinorhizobium meliloti can live as a soil saprophyte, and can engage in a nitrogen fixing symbiosis with plant roots. To succeed in such diverse environments, the bacteria must continually adjust gene expression. Transcriptional plasticity in eubacteria is often mediated by alternative sigma factors interacting with core RNA polymerase. The S. meliloti genome encodes 14 of these alternative sigmas, including two putative RpoH (heat shock) sigmas. We used custom Affymetrix Symbiosis Chips to characterize the global transcriptional response of S. meliloti rpoH1, rpoH2 and rpoH1 rpoH2 mutants during heat shock and stationary phase growth. Under these conditions, expression of over 300 genes is dependent on rpoH1 and rpoH2. Gene expression profiling of Sinorhizobium meliloti Rm1021 and its isogenic rpoH1, rpoH2, and rpoH1rpoH2 mutants, subjected to heat shock or stationary phase growth, was performed using custom Affymetrix GeneChips

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The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (SyrM)

Project description:We characterized transcriptomes of a strain overexpressing syrM. Our work shows that the syrM transcriptome shares similar gene expression changes to the syrA and nodD3 transcriptomes and that nodD3 and syrA may be the only targets directly activated by SyrM. We propose that most of the gene expression changes observed when nodD3 is overexpressed are due to NodD3 activation of syrM expression, which in turn stimulates SyrM activation of syrA expression. The subsequent increase in SyrA abundance alters activity of the ChvI-ExoS-ExoR circuit, resulting in broad changes in gene expression.

2015-03-31 | GSE61525 | GEO

The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (SyrA)

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Defining the transcriptional regulon of ChvI in Caulobacter crescentus

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2021-04-01 | GSE168965 | GEO

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