Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Deregulated expression programs in the hypomorphic Dnmt3b mutant mice

ABSTRACT: The function of Dnmt3b, of which deregulated activity is linked to several human pathologies, was studied using Dnmt3b hypomorphic mutant mice with reduced catalytic activity. Microarray analysis of deregulated expression programs in the hypomorphic Dnmt3b mutant mice (m3/m24) was combined to an analysis of the molecular mechanisms involved in the illegitimate activation of a specific set of genes. Mouse embryonic fibroblasts and thymus tissues isolated from 12.5 dpc and 18.5 dpc, respectively, wild-type and hypomorphic embryos (129SvJae x C57BL/6 hybrid genetic background). Total RNA was isolated using RNeasy Mini kit (QIAGEN), amplified, labeled, and hybridized following a previously described protocol (Le Brigand et al., Nuc Acid Res, 2006). Total RNA was coupled with Cyanine 3 or 5 and then hybridized in competition with reference RNA composed of a pool of total RNA isolated from wild-type thymus or MEF cells (thymus, n=5; MEF, n=5); two dye-swap were realized leading to the analysis of 18 microarrays for thymus (2 microarrays were excluded for unsufficient quality) and 20 for MEF cells. Arrays were then scanned with Agilent G2565AA Microarray Scanner (Agilent Technologies).

ORGANISM(S): Mus musculus

SUBMITTER: Jérome Rollin

PROVIDER: E-GEOD-19597 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues.

Velasco Guillaume G Hubé Florent F Rollin Jérôme J Neuillet Damien D Philippe Cathy C Bouzinba-Segard Haniaa H Galvani Angélique A Viegas-Péquignot Evani E Francastel Claire C

Proceedings of the National Academy of Sciences of the United States of America 20100503 20

Methylation of cytosine residues within the CpG dinucleotide in mammalian cells is an important mediator of gene expression, genome stability, X-chromosome inactivation, genomic imprinting, chromatin structure, and embryonic development. The majority of CpG sites in mammalian cells is methylated in a nonrandom fashion, raising the question of how DNA methylation is distributed along the genome. Here, we focused on the functions of DNA methyltransferase-3b (Dnmt3b), of which deregulated activity ...[more]

PMID: 20439742

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Dataset Information

Deregulated expression programs in the hypomorphic Dnmt3b mutant mice

Publications

Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues.

Similar Datasets

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