Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Steady state +/- Gly


ABSTRACT: Cells were grown to early log phase (OD600 ~ 0.2) at 30oC in 1 liter of minimal B medium (Cherest, H., and Surdin-Kerjan, Y. (1992) Genetics 130, 51-58) with or without supplementation with 10 mM glycine and harvested. All cells were harvested by rapid centrifugation at room temperature then flash frozen in liquid nitrogen. Arrays compared expression of Gly- cells to the Gly+ controls. Strain BY4741 was the wild type and several deletion strains were also used. Keywords: parallel sample

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Andy Bognar 

PROVIDER: E-GEOD-1553 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Transcriptional regulation of the one-carbon metabolism regulon in Saccharomyces cerevisiae by Bas1p.

Subramanian Mohan M   Qiao Wen-ben WB   Khanam Nurussaba N   Wilkins Olivia O   Der Sandy D SD   Lalich Jonathan D JD   Bognar Andrew L AL  

Molecular microbiology 20050701 1


The mechanisms mediating responses to glycine withdrawal in budding yeast were studied using a genome-wide profiling approach. A striking pattern of repressed expression of genes with an enrichment for those involved in one-carbon metabolism and AMP biosynthesis was revealed. Sequence analysis of the promoters for the most severely repressed genes identified a conserved sequence, TGACTC, a known binding site for the transcription factors Gcn4p and Bas1p. Loss of BAS1 abolished or significantly r  ...[more]

Similar Datasets

2010-10-08 | E-GEOD-1554 | biostudies-arrayexpress
2004-07-17 | GSE1553 | GEO
2004-07-17 | GSE1554 | GEO
2010-07-01 | E-GEOD-4445 | biostudies-arrayexpress
2010-04-21 | E-GEOD-19597 | biostudies-arrayexpress
2010-06-09 | E-GEOD-996 | biostudies-arrayexpress
2010-06-11 | E-GEOD-5911 | biostudies-arrayexpress
2010-06-12 | E-GEOD-5912 | biostudies-arrayexpress
2010-05-26 | E-GEOD-9875 | biostudies-arrayexpress
2012-12-31 | E-GEOD-40095 | biostudies-arrayexpress