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DNA methylation in progenitor cells: expression study

ABSTRACT: We surveyed DNA methylation profiles of all human RefSeq promoters in relation to gene expression and differentiation in adipose tissue, bone marrow and muscle mesenchymal progenitors, as well as in bone marrow-derived hematopoietic progenitors. We unravel strongly overlapping DNA methylation profiles between adipose stem cells (ASCs), bone marrow mesenchymal stem cells (BMMSCs) and muscle progenitor cells (MPCs), while hematopoietic progenitor cells (HPCs) are more epigenetically distant from MSCs seen as a whole. Differentiation resolves a fraction of methylation patterns common to MSCs, generating epigenetic divergence. RNA was isolated from MSCs isolated from various tissues and from differentiated cells, and hybridized onto Illumina expression arrays. 2-3 replicates per cell type. The supplementary file 'GSE19773_non-normalized.txt' contains the non-normalized data for Samples GSM493884-GSM493894.

ORGANISM(S): Homo sapiens

SUBMITTER: Philippe Collas

PROVIDER: E-GEOD-19773 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Promoter DNA methylation patterns of differentiated cells are largely programmed at the progenitor stage.

Sørensen Anita L AL Jacobsen Bente Marie BM Reiner Andrew H AH Andersen Ingrid S IS Collas Philippe P

Molecular biology of the cell 20100421 12

Mesenchymal stem cells (MSCs) isolated from various tissues share common phenotypic and functional properties. However, intrinsic molecular evidence supporting these observations has been lacking. Here, we unravel overlapping genome-wide promoter DNA methylation patterns between MSCs from adipose tissue, bone marrow, and skeletal muscle, whereas hematopoietic progenitors are more epigenetically distant from MSCs as a whole. Commonly hypermethylated genes are enriched in signaling, metabolic, and ...[more]

PMID: 20410135

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