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Transcription profiling of initial stages of metanephric development in mouse and rat

ABSTRACT: In this study we identify molecules with highly restricted expression patterns during the initial stages of metanephric development, when the ureteric bud has entered the metanephric mesenchyme and initiated branching morphogenesis. Using the Affymetrix Mouse Genome 430 2.0 Array, we compare gene expression patterns in ureteric bud tips, stalks and metanephric mesenchymes from mouse E12.5 embryos. To identify conserved molecular pathways, we also analyze transcriptional profiles in rat E13.5 ureteric buds and metanephric mesenchymes using the Affymetrix Rat Genome U34 Set. Taken together, these data sets help to identify conserved and highly localized transcripts in the metanephric kidney.

ORGANISM(S): Mus musculus

SUBMITTER: Kai Martin Schmidt-Ott

PROVIDER: E-GEOD-1983 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Novel regulators of kidney development from the tips of the ureteric bud.

Schmidt-Ott Kai M KM Yang Jun J Chen Xia X Wang Howard H Paragas Neal N Mori Kiyoshi K Li Jau-Yi JY Lu Benson B Costantini Frank F Schiffer Mario M Bottinger Erwin E Barasch Jonathan J

Journal of the American Society of Nephrology : JASN 20050525 7

Mammalian nephrogenesis depends on the interaction between the ureteric bud and the metanephric mesenchyme. As the ureteric bud undergoes branching and segmentation, the stalks differentiate into the collecting system of the mature kidney, while the tip cells interact with the adjacent cells of the metanephric mesenchyme, inducing their conversion into nephrons. This induction is mediated by secreted factors. For identifying novel mediators, the tips of the ureteric tree were isolated and microa ...[more]

PMID: 15917337

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