Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of rat ureteric buds treated with either heregulin, pleiotrophin or conditioned medium from metanephric mesenchymal cell culture

ABSTRACT: Genes ontologically classified as proliferative and developmental were down-regulated by HRG, whereas those involved in transport were up-regulated. For example, the mRNA for the GDNF receptors, GFRa1 and ret9 were down-regulated while the mRNA for collecting duct transporters, such as urea transporter2, aquaporin3, sodium-hydrogen exchanger2 were up-regulated in HRG-treated UBs compared with UBs grown in the presence of branch-promoting factors Experiment Overall Design: Total RNA was extracted from 3 isolated ureteric buds in culture per one treatment condition (1 sample). Three samples (biological replicates) were used for one condition.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Hiroyuki Sakurai

PROVIDER: E-GEOD-3394 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Heregulin induces glial cell line-derived neurotrophic growth factor-independent, non-branching growth and differentiation of ureteric bud epithelia.

Sakurai Hiroyuki H Bush Kevin T KT Nigam Sanjay K SK

The Journal of biological chemistry 20050923 51

We have purified a protein present in a conditioned medium derived from the metanephric mesenchyme that supports non-branching growth and epithelial differentiation of the isolated ureteric bud (UB) independent of glial cell line-derived neurotrophic growth factor (GDNF). By sequential liquid chromatography, together with protein microsequencing, the protein was identified as heregulin (HRG)alpha. The addition of recombinant HRG to the isolated UB grown in three-dimensional culture confirmed the ...[more]

PMID: 16183643

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Project description:Severe hyposalivation often results from Sjögren’s syndrome or radiation therapy for head and neck cancer. Devastating consequences of hyposalivation, such as rampant dental caries and persistent oral candidiasis, compromise the quality of life in those patients. Clinical management for dry mouth typically involves simple palliative methods or secretagogues, which are designed to stimulate saliva secretion from residual salivary acinar cells present in the glands. However, direct interventions in chronic dryness have yet to be employed in the clinical setting. Despite numerous studies on salivary gland regeneration, the molecular basis governing stem cell transdifferentiation into salivary epithelial precursors (SEP) is largely unknown. Our previously published study clearly indicated mouse bone marrow-derived mesenchymal stem cells (BM-MSCs) can be differentiated into SEP in vitro when co-cultured with isolated primary salivary gland cells without cell-to-cell contact. Our current study utilized iTRAQ-LS-MS/MS-based quantitative proteomics to profile key regulatory factors involved in mouse MSC-to-SEP conversion. We identified 280 differentially expressed proteins in BM-MSCs over the course of 7 days of co-culture. Interestingly, protein expression of salivary transcription factors (STFs), such as transcription factor E2a (TCF3), high mobility group protein 20B (HMG20b), and ankyrin repeat domain-containing protein 56 (ANKRD56) were increased in a time-dependent manner. Notably, pancreas specific transcription factor 1a (PTF1α), muscle, intestine and stomach expression-1 (MIST-1) and achaete-scute complex homolog 3(ASCL3) were newly induced over time in differentiated MSCs. We also identified the expression of Ptf1α in the mouse salivary glands for the first time and verified its expression in independent batches of co-cultured MSCs by western blotting. Furthermore, simulation of the molecular network involving the identified STFs has demonstrated evidence for their perspective roles in salivary gland development during glandular maturation. Thus, our study provides the first extensive proteomic profile of MSC-to-SEP transdifferentiation and identifies novel STFs that may be critical for this process.

Dataset Information

Transcription profiling of rat ureteric buds treated with either heregulin, pleiotrophin or conditioned medium from metanephric mesenchymal cell culture

Publications

Heregulin induces glial cell line-derived neurotrophic growth factor-independent, non-branching growth and differentiation of ureteric bud epithelia.

Similar Datasets

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