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Unknown,Transcriptomics,Genomics,Proteomics

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Interleukin-16-knock-out bone-marrow derived macrophages response to Escherichia coli lipopolysaccharide (LPS)

ABSTRACT: We have employed whole genome microarray expression profiling to identify differently expressed genes following stimulation of interleukin-16-knock-out bone-marrow derived macrophages with Escherichia coli lipopolysaccharide (LPS). Macrophages were stimulated with LPS (100 ng/ml) for 6 hours and a signature was identified that distinguished between infected and control samples. Expression of several genes from this signature was quantified in the same RNA samples by real-time PCR, confirming the predicted macrophage response pattern. Gene expression in macrophages was measured at 6 hours after stimulation Three independent experiments were performed.

ORGANISM(S): Mus musculus

SUBMITTER: Benoit Desnues

PROVIDER: E-GEOD-20208 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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