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Comparison of the CcpA and CovR Transcriptomes in Group A Streptococcus

ABSTRACT: The goal of this study were to compare the transcriptoms of CcpA and CovR in group A Streptococcus. Isogenic mutant strains were created in which CcpA and CovR were inactivated alone and in combination. The transcriptomes of the four strains (wild-type, CcpA mutant, CovR mutant, and ccpA-covR double mutant) were then compared. Four biological replicates of each strain were grown to the mid-exponential and stationary phases of growth in THY. Cells were harvested, RNA isolated, and converted to cDNA. cDNA was hybridized to a custom-made Affymetrix GeneChip that contains 100% of the ORFs of the wild-type strain MGAS2221.

ORGANISM(S): Streptococcus sp. 'group A'

SUBMITTER: Richard Brennan

PROVIDER: E-GEOD-20212 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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