Ontology highlight
ABSTRACT:
ORGANISM(S): Caenorhabditis elegans
SUBMITTER: Noah Welker
PROVIDER: E-GEOD-20336 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Welker Noah C NC Pavelec Derek M DM Nix David A DA Duchaine Thomas F TF Kennedy Scott S Bass Brenda L BL
RNA (New York, N.Y.) 20100330 5
Years after the discovery that Dicer is a key enzyme in gene silencing, the role of its helicase domain remains enigmatic. Here we show that this domain is critical for accumulation of certain endogenous small interfering RNAs (endo-siRNAs) in Caenorhabditis elegans. The domain is required for the production of the direct products of Dicer, or primary endo-siRNAs, and consequently affects levels of downstream intermediates, the secondary endo-siRNAs. Consistent with the role of endo-siRNAs in si ...[more]