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Transcription profiling of human CD34 cells isolated from cord blood or peripheral blood transduced with AML1-ETO or empty vector (MIGR1)

ABSTRACT: Human CD34 cells isolated from cord blood or peripheral blood transduced with AML1-ETO or empty vector (MIGR1).

ORGANISM(S): Homo sapiens

SUBMITTER: Agnes Viale

PROVIDER: E-GEOD-2049 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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AML1-ETO fusion protein up-regulates TRKA mRNA expression in human CD34+ cells, allowing nerve growth factor-induced expansion.

Mulloy James C JC Jankovic Vladimir V Wunderlich Mark M Delwel Ruud R Cammenga Jorg J Krejci Ondrej O Zhao Hui H Valk Peter J M PJ Lowenberg Bob B Nimer Stephen D SD

Proceedings of the National Academy of Sciences of the United States of America 20050224 11

The AML1-ETO fusion protein, generated by the t(8;21) in acute myeloid leukemia (AML), exerts dominant-negative functions and a variety of gains of function, including a positive effect on the growth of primary human CD34+ hematopoietic stem/progenitor cells. We now show that AML1-ETO expression up-regulates the level of TRKA mRNA and protein in these cells and that AML1-ETO-expressing CD34+ hematopoietic cells grown in the presence of five early-acting hematopoietic cytokines further proliferat ...[more]

PMID: 15731354

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