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Karrikin responses in Arabidopsis thaliana seed


ABSTRACT: Karrikins promote seed germination in Arabidopsis thaliana. Completion of germination (protrusion of the radicle) is not observed until ~72 h in dormant wildtype seed under these conditions. We used microarrays to examine karrikin-induced transcriptional changes after 24 h of imbibition. Transcriptional changes may indicate events leading to karrikin-induced germination or karrikin-specific markers. Primary dormant seed of wildtype (which can germinate and is promoted by KAR1) and ga1-3 (which cannot germinate, and is not promoted by KAR1) were imbibed on water-agar or 1 uM KAR1 in the light at 20 C and assayed for gene expression after 24 h. Three or four biological replicates per genotype/treatment.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Steven Smith 

PROVIDER: E-GEOD-20556 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Karrikins enhance light responses during germination and seedling development in Arabidopsis thaliana.

Nelson David C DC   Flematti Gavin R GR   Riseborough Julie-Anne JA   Ghisalberti Emilio L EL   Dixon Kingsley W KW   Smith Steven M SM  

Proceedings of the National Academy of Sciences of the United States of America 20100329 15


Karrikins are a class of seed germination stimulants identified in smoke from wildfires. Microarray analysis of imbibed Arabidopsis thaliana seeds was performed to identify transcriptional responses to KAR(1) before germination. A small set of genes that are regulated by KAR(1), even when germination is prevented by the absence of gibberellin biosynthesis or light, were identified. Light-induced genes, putative HY5-binding targets, and ABRE-like promoter motifs were overrepresented among KAR(1)-  ...[more]

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