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MRNA profiling of genetically matched ESCs and iPSCs

ABSTRACT: Induced pluripotent stem cells (iPSCs) can be generated by enforced expression of defined transcription factors in somatic cells. It remains controversial whether iPSCs are equivalent to blastocyst-derived embryonic stem cells (ESCs). Using genetically matched cells, we found that the overall mRNA expression patterns of these cell types are indistinguishable with the exception of a few transcripts encoded on chromosome 12qF1. iPSCs were derived from somatic tissues of chimeric mice generated with ESCs that carry an inducible cassette encoding for the reprogramming factors myc, Klf4, Oct4 and Sox2.

ORGANISM(S): Mus musculus

SUBMITTER: Matthias Stadtfeld

PROVIDER: E-GEOD-20572 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells.

Stadtfeld Matthias M Apostolou Effie E Akutsu Hidenori H Fukuda Atsushi A Follett Patricia P Natesan Sridaran S Kono Tomohiro T Shioda Toshi T Hochedlinger Konrad K

Nature 20100425 7295

Induced pluripotent stem cells (iPSCs) have been generated by enforced expression of defined sets of transcription factors in somatic cells. It remains controversial whether iPSCs are molecularly and functionally equivalent to blastocyst-derived embryonic stem (ES) cells. By comparing genetically identical mouse ES cells and iPSCs, we show here that their overall messenger RNA and microRNA expression patterns are indistinguishable with the exception of a few transcripts encoded within the imprin ...[more]

PMID: 20418860

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Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells

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