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Expressoin data from iPSC with different cell of origin

ABSTRACT: Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly and functionally similar. We use expresson profiling to determine differnces in iPSCs with different cell of origin. Using a secondary system, we derived iPSCs from tail tip-derived fibroblasts (TTF-iPSC), splenic B cells (B-iPSC), bone marrow-derived granulocytes (Gra-iPSC) , skeletal muscle precursors (SMP-iPSC) and T cells (T-iPSC).

ORGANISM(S): Mus musculus

SUBMITTER: Jose Polo

PROVIDER: E-GEOD-22043 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells.

Polo Jose M JM Liu Susanna S Figueroa Maria Eugenia ME Kulalert Warakorn W Eminli Sarah S Tan Kah Yong KY Apostolou Effie E Stadtfeld Matthias M Li Yushan Y Shioda Toshi T Natesan Sridaran S Wagers Amy J AJ Melnick Ari A Evans Todd T Hochedlinger Konrad K

Nature biotechnology 20100719 8

Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly and functionally similar. Here we show that iPSCs obtained from mouse fibroblasts, hematopoietic and myogenic cells exhibit distinct transcriptional and epigenetic patterns. Moreover, we demonstrate that cellular origin influences the in vitro differentiation potentials of ...[more]

PMID: 20644536

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