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Unknown,Transcriptomics,Genomics,Proteomics

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Genetic analysis of the genome wide role of G-proteins in rice metabolism

ABSTRACT: The current study reports a genome-wide transcriptional profiling of the only known GM-NM-1 mutants in rice (RGA1). Total RNA was isolated from the leaves of 25 day old rice d1 (GM-NM-1) mutant (HO-541) and its corresponding wild type (Nipponbare) grown on B-5 agar media.Total RNAs from the rice GM-NM-1 mutant and wild type were first converted into cDNAs, from which labeled cRNAs were generated with Cy3 and Cy5 dyes and hybridized with 44K 60-mer whole genome rice microarrays on glass slides.The slides were washed and scanned on an Agilent scanner (G2565B) at 100 % laser power and data extracted with Agilent Feature Extraction software (version 9.1). The experiment was repeated with a biological-cum-technical replicate, where total RNA samples from a fresh batch of rice (mutant and WT) plants were labeled with opposite dyes (dye-swap) and used for microarray analysis.

ORGANISM(S): Oryza sativa

SUBMITTER: Nandula Raghuram

PROVIDER: E-GEOD-20925 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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