Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional Response to Iron Limitation in Pelagibacter ubique


ABSTRACT: We investigated the gene expression responses of Candidatus Pelagibacter ubique cultures to iron limitation. Differential expression was observed for genes in iron acquisition and incorporation operons. SfuC in particular was 16 times higher in iron-limited cultures and encodes a periplasmic iron-binding protein. Six natural seawater cultures were amended with minimal nutrients and inoculated with P. ubique. Close to maximum cell density, all carboys were supplemented with 100 nM ferrichrome (an iron-chelating siderophore) and three carboys were additionally supplemented with 1 µM FeCl3. Each of the six carboys was sampled for microarray analyses one, two, and eleven days after the ferrichrome addition.

ORGANISM(S): Candidatus Pelagibacter ubique HTCC1062

SUBMITTER: Daniel Smith 

PROVIDER: E-GEOD-20962 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptional and translational regulatory responses to iron limitation in the globally distributed marine bacterium Candidatus pelagibacter ubique.

Smith Daniel P DP   Kitner Joshua B JB   Norbeck Angela D AD   Clauss Therese R TR   Lipton Mary S MS   Schwalbach Michael S MS   Steindler Laura L   Nicora Carrie D CD   Smith Richard D RD   Giovannoni Stephen J SJ  

PloS one 20100505 5


Iron is recognized as an important micronutrient that limits microbial plankton productivity over vast regions of the oceans. We investigated the gene expression responses of Candidatus Pelagibacter ubique cultures to iron limitation in natural seawater media supplemented with a siderophore to chelate iron. Microarray data indicated transcription of the periplasmic iron binding protein sfuC increased by 16-fold, and iron transporter subunits, iron-sulfur center assembly genes, and the putative f  ...[more]

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