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Transcriptional and translational regulatory responses to iron limitation in the globally distributed marine bacterium Candidatus Pelagibacter ubique

ABSTRACT: Investigation of the gene expression responses of Candidatus Pelagibacter ubique cultures to iron limitation in natural seawater media supplemented with a siderophore to chelate iron. Quantitative peptide mass spectrometry revealed that sfuC protein abundance increased 27-fold, despite an average decrease of 59% across the global proteome.

OTHER RELATED OMICS DATASETS IN: PRJNA124525

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Velos

ORGANISM(S): Candidatus Pelagibacter Ubique (ncbitaxon:198252)

SUBMITTER: Stephen J. Giovannoni

PROVIDER: MSV000085445 | MassIVE | Mon May 18 21:57:00 BST 2020

REPOSITORIES: MassIVE

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Transcriptional and translational regulatory responses to iron limitation in the globally distributed marine bacterium Candidatus pelagibacter ubique.

Smith Daniel P DP Kitner Joshua B JB Norbeck Angela D AD Clauss Therese R TR Lipton Mary S MS Schwalbach Michael S MS Steindler Laura L Nicora Carrie D CD Smith Richard D RD Giovannoni Stephen J SJ

PloS one 20100505 5

Iron is recognized as an important micronutrient that limits microbial plankton productivity over vast regions of the oceans. We investigated the gene expression responses of Candidatus Pelagibacter ubique cultures to iron limitation in natural seawater media supplemented with a siderophore to chelate iron. Microarray data indicated transcription of the periplasmic iron binding protein sfuC increased by 16-fold, and iron transporter subunits, iron-sulfur center assembly genes, and the putative f ...[more]

PMID: 20463970

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