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Hog1 mutant 4 hours exposure to congo red (cell wall chip)


ABSTRACT: S. cerevisiae hog1 mutant strain was grown on YEPD. For Congo Red experiments, yest cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with Congo Red to a final concentration of 30 µg/ml. Cells were collected at 4hours of growth, frozen at -80 °C and processed for RNA extraction.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Rosa Pérez-Díaz 

PROVIDER: E-GEOD-2107 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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