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Effect of clp1 induction on b-dependent gene regulation

ABSTRACT: The clp1 gene is a b-regulated gene that is required for clamp formation during the biotrophic growth phase of U. maydis. Clp1 expression blocks b-dependent filament formation and cell cycle arrest if co-induced with the b-heterodimer. Strains AB33 (control) and UMS84 were grown for the times indicated in array medium containing 3 g/l nitrate and 1% arabinose for the induction of nar1P- (bE1/bW2) and crg1P- (clp1) controlled gene expression. Samples were taken from two biological replicates each.

ORGANISM(S): Ustilago maydis

SUBMITTER: Jörg Kämper

PROVIDER: E-GEOD-21119 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription factor Rbf1 is the master regulator for b-mating type controlled pathogenic development in Ustilago maydis

Project description:This SuperSeries is composed of the following subset Series: GSE18750: Controlled expression of compatible and incompatible combinations of Ustilago maydis b-mating type locus genes bE and bW GSE18754: Effect of rbf1 deletion during controlled expression of of Ustilago maydis b-mating type locus genes bE1 and bW2 GSE18756: Rbf1 induced gene expression in Ustilago maydis Refer to individual Series

2010-06-01 | E-GEOD-18881 | biostudies-arrayexpress

Controlled expression of compatible and incompatible combinations of Ustilago maydis b-mating type locus genes bE and bW

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2010-06-01 | E-GEOD-18750 | biostudies-arrayexpress

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Project description:The clp1 gene is a b-regulated gene that is required for clamp formation during the biotrophic growth phase of U. maydis. Interaction studies demonstrated that Clp1 imnteracts with Rbf1, the central regulator of pathogenciity. To exclude b-dependent effects the experiments were performed in a b-null background to analyze clp1-depndent effects on rbf controlled gene regulation Strains UKH156 (nitrate inducible Rbf1 expression) and UKH164 (arabinose-inducible Clp1 and and nitrate-inducible Rbf1 expression) were grown for 12h in liquid array medium/nitrate/arabinose.

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2010-06-01 | E-GEOD-18754 | biostudies-arrayexpress

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2010-06-12 | E-GEOD-6039 | biostudies-arrayexpress

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2009-09-29 | E-GEOD-16634 | biostudies-arrayexpress

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2019-04-04 | E-MTAB-7460 | biostudies-arrayexpress

RNAseq of Ustilago maydis with inducible clp1 and Δcib1

Project description:Ustilago maydis strain JB1 was modified to include an arabinose inducible clp1 in the ip-locus and clb1 was deleted.

2019-04-04 | E-MTAB-7463 | biostudies-arrayexpress

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Project description:The response of P. putida CSV86 in response to preferential carbon source and varying nitrogen levels was studied in this experiment. The culture was grown in minimal medium with either naphthalene (0.1%) alone or along with glucose (0.25%) as carbon source and different concentration of ammonium nitrate. For nitrogen limiting 0.1% NH4N03 was added in the medium and for nitrogen rich 1% NH4N03 was used. As control CSV86 was also grown in 1X Luria Broth with glucose as additional carbon source. Overall it was seen that the strain could grow efficiently in varying nitrogen conditions by adapting or regulating their metabolism without compromising on the growth rate. To ascertain how the cells were coping with the nitrogen stress, gene expression by microarray was performed. RNA extraction was done using Qiagen RNeasy minikit (Germany). Standard Affymetrix protocol was followed for hybridization with GeneChip P. aeruginosa Genome Array supplied by Affymetrix as GeneChip for P. putida were not supplied by any company.

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