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Genome-wide methylation analysis of esophageal squamous cell carcinoma

ABSTRACT: Genome-wide methylation analysis was performed by methylated DNA immunoprecipitation-CGI microarray analysis. A pool of four cancer samples without lymph node metastasis, two pools of four cancer samples with lymph node metastasis, and three metastatic lymph nodes were prepared from the patients, and analyzed by MeDIP-CGI microarray.

ORGANISM(S): Homo sapiens

SUBMITTER: Satoshi Yamashita

PROVIDER: E-GEOD-21238 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification and validation of DNA methylation markers to predict lymph node metastasis of esophageal squamous cell carcinomas.

Gyobu Ken K Yamashita Satoshi S Matsuda Yasunori Y Igaki Hiroyasu H Niwa Tohru T Oka Daiji D Kushima Ryoji R Osugi Harushi H Lee Shigeru S Suehiro Shigefumi S Ushijima Toshikazu T

Annals of surgical oncology 20101102 4

<h4>Background</h4>The presence of lymph node metastasis in esophageal squamous cell carcinoma (ESCC) patients is a critical factor for decision of treatment strategy. However, there have been no molecular markers to assess lymph node metastasis. In this study, we aimed to identify CpG islands (CGIs) whose DNA methylation statuses are associated with the presence of lymph node metastasis.<h4>Materials and methods</h4>A total of 96 ESCCs were divided into a screening set (n = 48) and a validation ...[more]

PMID: 21042947

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