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Genome-wide measurement of mRNA lifetime in Escherichia coli

ABSTRACT: We measured the mRNA abundance in E.coli using RNAseq to calculate mRNA lifetimes. The data is used in support of a larger paper on the proteome and transcriptome of E.coli. Comparison of mRNA abundance over time, after the addition of transcription inhibitor, rifampicin. Center: Harvard University

ORGANISM(S): Escherichia coli

SUBMITTER: Huiyi Chen

PROVIDER: E-GEOD-21341 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells.

Taniguchi Yuichi Y Choi Paul J PJ Li Gene-Wei GW Chen Huiyi H Babu Mohan M Hearn Jeremy J Emili Andrew A Xie X Sunney XS

Science (New York, N.Y.) 20100701 5991

Protein and messenger RNA (mRNA) copy numbers vary from cell to cell in isogenic bacterial populations. However, these molecules often exist in low copy numbers and are difficult to detect in single cells. We carried out quantitative system-wide analyses of protein and mRNA expression in individual cells with single-molecule sensitivity using a newly constructed yellow fluorescent protein fusion library for Escherichia coli. We found that almost all protein number distributions can be described ...[more]

PMID: 20671182

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genome-wide measurement of mRNA lifetime in Escherichia coli

Project description:We measured the mRNA abundance in E.coli using RNAseq to calculate mRNA lifetimes. The data is used in support of a larger paper on the proteome and transcriptome of E.coli.

2010-07-30 | GSE21341 | GEO

Real-time quantitative PCR analysis of gene expression in the cerebellum of MeCP2 A140V mutant mice

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Real-time quantitative PCR analysis of the expression of chromatin modification enzyme genes in 4 week-old male MeCP2 A140V mutant mouse cerebellum

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2015-02-01 | E-GEOD-35689 | biostudies-arrayexpress

Real-time quantitative PCR analysis of the expression of chromatin modification enzyme genes in 2 week-old male MeCP2 A140V mutant mouse cerebellum

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Real-time quantitative PCR analysis of gene expression in the cortex of MeCP2 A140V mutant mice

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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2015-02-01 | E-GEOD-35767 | biostudies-arrayexpress

Real-time quantitative PCR analysis of the expression of chromatin modification enzyme genes in 4 week old male MeCP2 A140V mutant mouse brain cortex

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2015-02-01 | E-GEOD-35768 | biostudies-arrayexpress

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