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Baculovirus-encoded protein expression for epigenomic profiling in Drosophila cells

ABSTRACT: Baculovirus-encoded proteins can be efficiently produced following infection of different Drosophila cell lines. Epitope-tagged proteins produced in baculovirus-infected cells provide genome-wide profiles of the histone variant H2Av that are comparable to those produced by stably transformed cell lines. Keywords: Chromatin affinity-purification on microarray All experiments were done using strepavidin or FLAG pulldown DNA cohybridized with total input DNA to the same array. Two channels per array, Cy5 and Cy3, were used in each experiment.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Jorja Henikoff

PROVIDER: E-GEOD-21416 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Baculovirus-encoded protein expression for epigenomic profiling in Drosophila cells.

Bryson Terri D TD Weber Christopher M CM Henikoff Steven S

Fly 20100701 3

The expression and genome-wide mapping of epitope-tagged DNA- and chromatin-binding proteins in cultured cells has become a powerful strategy for epigenome characterization, especially in Drosophila, where cell lines derived from numerous tissues are now available. However this strategy relies on establishing transfected cell lines, which is time-consuming and introduces variability. Here we show that baculovirus-encoded proteins can be efficiently produced following infection of Drosophila cell ...[more]

PMID: 20495356

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