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The nucleosomal barrier to promoter escape by RNA Polymerase II is overcome by the chromatin remodeler Chd1 [NimbleGen]

ABSTRACT: We use a metabolic labeling strategy for directly measuring nucleosome turnover to determine the role of chromatin remodeler Chd1 on chromatin dynamics in mouse embryonic fibroblasts expressing with wildtype-Chd1 and dominant negative K510R-Chd1. We find that expression of K510R-Chd1 decreases nucleosome turnover at the promoter and increases turnover within the gene body. 10 Illumina sequencing samples and 4 Nimblegen array samples

ORGANISM(S): Mus musculus

SUBMITTER: Jorja Henikoff

PROVIDER: E-GEOD-52347 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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