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Gene expression profiles of an E10.5 ureteric duct cell (GUDMAP Series_id: 40)

ABSTRACT: The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. HoxB7-GFP transgenic mice were utilized to isolate the ureteric duct cells from E10.5 embryonic kidneys. The ureteric duct cells were isolated from embryos using microdissection, trypsin treatment and FACS. The RNA was isolated from purified ureteric duct cells and the gene expression profiles were determined by microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Eric Brunskill

PROVIDER: E-GEOD-21584 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profiles of E10.5 ureteric bud cells. (GUDMAP Series_id: 39)

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Transcription profiling of initial stages of metanephric development in mouse and rat

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2017-08-15 | GSE93267 | GEO

Gene expression profiles of the cap mesenchyme & renal vesicle in the neonatal kidney (GUDMAP Series_id: 28)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. At E18.5, pups were isolated from CRYM-BAC transgenic mice and fostered to CD-1 mice. Cap mesenchyme cells were isolated from the kidney by fluorescent activated cell sorting (FACS) on postnatal days 0, 1, 2, 3, and 4. RNA was isolated from FACS sorted cells and the gene expression profiles were determined by microarrays.

2010-07-16 | E-GEOD-17139 | biostudies-arrayexpress

Gene expression profiles of the adult capsular cell (GUDMAP Series_id: 36)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. CD-1 mice were utilized to isolate the capsule cells from the adult kidneys. The capsule cells were isolated from the kidney using microdissection treatment. RNA was isolated and the gene expression profiles were determined by microarrays.

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