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Lactobacillus johnsonii NCC comparative genomics with L. iatae

ABSTRACT: Whole genome DNA microarray designed for the probiotic L. johnsonii strain NCC533 was used for comparative genomic hybridization (CGH) of L. johnsonii ATCC 33200T, L. johnsonii BL261, L. gasseri ATCC 33323T and L. iatae BL263 (CECT 7394T). In these experiments, the fluorescence ratio distributions obtained with L. iatae and L. gasseri showed characteristic inter-species profiles. The percentage of conserved L. johnsonii NCC533 genes was about 83% in the L. johnsonii strains comparisons and decreased to 51% and 47% for L. iatae and L. gasseri, respectively. These results confirmed the separate status of L. iatae from L. johnsonii at the level of species, and also that it is closer to L. johnsonii than L. gasseri. L. johnsonii, L. gasseri, and L. iatae strains were hybridized versus L. johnsonii NCC533, some with replicates

ORGANISM(S): Lactobacillus johnsonii

SUBMITTER: Bernard Berger

PROVIDER: E-GEOD-21627 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Characterization of a novel Lactobacillus species closely related to Lactobacillus johnsonii using a combination of molecular and comparative genomics methods.

Sarmiento-Rubiano Luz-Adriana LA Berger Bernard B Moine Déborah D Zúñiga Manuel M Pérez-Martínez Gaspar G Yebra María J MJ

BMC genomics 20100917

<h4>Background</h4>Comparative genomic hybridization (CGH) constitutes a powerful tool for identification and characterization of bacterial strains. In this study we have applied this technique for the characterization of a number of Lactobacillus strains isolated from the intestinal content of rats fed with a diet supplemented with sorbitol.<h4>Results</h4>Phylogenetic analysis based on 16S rRNA gene, recA, pheS, pyrG and tuf sequences identified five bacterial strains isolated from the intesti ...[more]

PMID: 20849602

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