Transcriptional profiling of flatfish (Solea senegalensis) spermatogenesis
Ontology highlight
ABSTRACT:
ORGANISM(S): Solea senegalensis
SUBMITTER: Josep Planas
PROVIDER: E-GEOD-21694 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Similar Datasets
Project description:The Senegalese sole (Solea senegalensis) is a marine flatfish of high economic value and a target species for aquaculture. Here, we used a transcriptomic approach to investigate changes in genes expressed in the Senegalese sole testis throughout spermatogenesis in wild-caught fish adapted to captivity. We identified approximately 400 genes that are differentially expressed during the progression of spermatogenesis and that participate in processes such as activation of the ubiquitin-proteasome system, sperm maturation and motility, cell adhesion or cytoskeletal remodeling. The results from this study contribute to our understanding of the molecular changes ocurring during spermatogenesis in the Senegalese sole. This study represents spermatogenesis in Solea senegalensis: mid versus late spermatogenesis. Total RNA from testes at different stages in spermatogenesis (early, mid, late and functional maturation) from F0 wild Senegalese sole (3-4 animals at each stage) was extracted using the RNeasy extraction kit (Qiagen) and treated with DNAse following the manufacturer’s instructions. Quantitative and qualitative analysis of total RNA was performed using the Agilent 2100 bioanalyzer. RNA samples from each stage were pooled and amplified, labelled and hybridized to a custom-made oligonucleotide microarray containing 5,087 Senegalese sole Unigene sequences. In brief, pooled testicular RNAs from each stage were amplified and the resulting cRNAs labelled with Cy3 and Cy5, respectively, mixed in equal amounts and hybridized to the microarray for 17 h at 60 ºC.
2010-05-07 | E-GEOD-21637 | biostudies-arrayexpress
Project description:The Senegalese sole (Solea senegalensis) is a marine flatfish of high economic value and a target species for aquaculture. Here, we used a transcriptomic approach to investigate changes in genes expressed in the Senegalese sole testis throughout spermatogenesis in wild-caught fish adapted to captivity. We identified approximately 400 genes that are differentially expressed during the progression of spermatogenesis and that participate in processes such as activation of the ubiquitin-proteasome system, sperm maturation and motility, cell adhesion or cytoskeletal remodeling. The results from this study contribute to our understanding of the molecular changes ocurring during spermatogenesis in the Senegalese sole. This study represents spermatogenesis in Solea senegalensis: early versus late spermatogenesis. Total RNA from testes at different stages in spermatogenesis (early, mid, late and functional maturation) from F0 wild Senegalese sole (3-4 animals at each stage) was extracted using the RNeasy extraction kit (Qiagen) and treated with DNAse following the manufacturer’s instructions. Quantitative and qualitative analysis of total RNA was performed using the Agilent 2100 bioanalyzer. RNA samples from each stage were pooled and amplified, labelled and hybridized to a custom-made oligonucleotide microarray containing 5,087 Senegalese sole Unigene sequences. In brief, pooled testicular RNAs from each stage were amplified and the resulting cRNAs labelled with Cy3 and Cy5, respectively, mixed in equal amounts and hybridized to the microarray for 17 h at 60 ºC. Each hybridization was performed at least in duplicate.
2010-05-07 | E-GEOD-21634 | biostudies-arrayexpress
Project description:The Senegalese sole (Solea senegalensis) is a marine flatfish of high economic value and a target species for aquaculture. Here, we used a transcriptomic approach to investigate changes in genes expressed in the Senegalese sole testis throughout spermatogenesis in wild-caught fish adapted to captivity. We identified approximately 400 genes that are differentially expressed during the progression of spermatogenesis and that participate in processes such as activation of the ubiquitin-proteasome system, sperm maturation and motility, cell adhesion or cytoskeletal remodeling. The results from this study contribute to our understanding of the molecular changes ocurring during spermatogenesis in the Senegalese sole. This study represents spermatogenesis in Solea senegalensis: functional mature versus late spermatogenesis. Total RNA from testes at different stages in spermatogenesis (early, mid, late and functional maturation) from F0 wild Senegalese sole (3-4 animals at each stage) was extracted using the RNeasy extraction kit (Qiagen) and treated with DNAse following the manufacturer’s instructions. Quantitative and qualitative analysis of total RNA was performed using the Agilent 2100 bioanalyzer. RNA samples from each stage were pooled and amplified, labelled and hybridized to a custom-made oligonucleotide microarray containing 5,087 Senegalese sole Unigene sequences. In brief, pooled testicular RNAs from each stage were amplified and the resulting cRNAs labelled with Cy3 and Cy5, respectively, mixed in equal amounts and hybridized to the microarray for 17 h at 60 ºC.
2010-05-07 | E-GEOD-21635 | biostudies-arrayexpress
Project description:In male teleosts, testicular steroids are essential hormones for the regulation of spermatogenesis and their production is regulated by pituitary gonadotropins. In the Senegalese sole (Solea senegalensis), an economically important flatfish with semi-cystic and asynchronous spermatogenesis, the endocrine mechanisms involved in the regulation of spermatogenesis, particularly regarding the production and regulation of testicular steroids, are not well understood.This study aimed at describing the transcriptomic changes taking place in the Senegalese sole testis in response to hCG stimulation in vivo. Gene expression analysis by microarray identified 90 differentially expressed genes in the testis in response to hCG administration, including genes potentially involved in steroidogenesis, progression of spermatogenesis and germ cell maturation and cytoskeletal organization. Our results provide evidence for the first time that key genes involved in the regulation of steroid production and spermatogenesis in the Senegalese sole testis are under gonadotropic control. Testes samples from saline- and hCG-injected male Senegalese sole (8 fish per group) were pooled separatedly. Pooled testicular RNAs from each group were amplified and the resulting cRNAs labelled with Cy3 and Cy5, respectively, mixed in equal amounts and hybridized to the microarray for 17 h at 60 ºC. Each hybridization was performed in duplicate.
2010-12-02 | E-GEOD-25733 | biostudies-arrayexpress
Project description:This SuperSeries is composed of the following subset Series: GSE17333: Oogenesis in Solea senegalensis: Vitellogenic versus mature ovaries GSE17334: Oogenesis in Solea senegalensis: vitellogenic versus atretic ovaries GSE17335: Oogenesis in Solea senegalensis: mature versus atretic ovaries GSE17336: Oogenesis in Solea senegalensis: Previtellogenic versus vitellogenic ovaries Refer to individual Series
2010-06-21 | E-GEOD-17337 | biostudies-arrayexpress
Project description:Transcriptionnal profile changes in ovary during the reproductive cycle of Solea senegalensis: from mature stage to atresia stage obtained at different times during the annual reproductive cycle. An oligo microarray based on ESTs sequenced from S. senegalensis previously designed (Cerdà et al., 2008, BMC genomics) was used. The two stages of ovary development were directly compared using three individuals for each stage (IR-Mt-1, IR-Mt-2, IR-Mt-3 for mature individuals and IR-At-1, IR-At-2, IR-At-3 for atretic individuals). For the experiment, the atretic stage was used as the control.
2010-06-21 | E-GEOD-17335 | biostudies-arrayexpress
Project description:Transcriptionnal profile changes in ovary during the reproductive cycle of Solea senegalensis: from vitellogenic stage to mature stage obtained at different times during the annual reproductive cycle. An oligo microarray based on ESTs sequenced from S. senegalensis previously designed (Cerdà et al., 2008, BMC genomics) was used. The two stages of ovary development were directly compared using three individuals for each stage (IR-Mt-1, IR-Mt-2, IR-Mt-3 for mature individuals and IR-Vt-1, IR-Vt-2, IR-Vt-3 for vitellogenic individuals). For the experiment, the mature stage was used as the control.
2010-06-21 | E-GEOD-17333 | biostudies-arrayexpress
Project description:Transcriptional profile changes in ovary during the reproductive cycle of Solea senegalensis: from vitellogenic stage to atresia stage obtained at different times during the annual reproductive cycle. An oligo microarray based on ESTs sequenced from S. senegalensis previously designed (Cerdà et al., 2008, BMC genomics) was used. The two stages of ovary development were directly compared using three individuals for each stage (IR-Vt-1, IR-Vt-2, IR-Vt-3 for vitellogenic individuals and IR-At-1, IR-At-2, IR-At-3 for atretic individuals). For the experiment, the atretic stage was used as the control.
2010-06-21 | E-GEOD-17334 | biostudies-arrayexpress
Project description:Transcriptionnal profile changes in ovary during the reproductive cycle of Solea senegalensis: from previtellogenic stage to vitellogenic stage obtained at different times during the annual reproductive cycle. An oligo microarray based on ESTs sequenced from S. senegalensis previously designed (Cerdà et al., 2008, BMC genomics) was used. The two stages of ovary development were directly compared using three individuals for each stage (IR-Pv-1, IR-Pv-2, IR-Pv-3 for previtellogenic individuals and IR-Vt-1, IR-Vt-2, IR-Vt-3 for vitellogenic individuals). For the experiment, the vitellogenic stage was used as the control.
2010-06-21 | E-GEOD-17336 | biostudies-arrayexpress
