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Elucidation of the expression profile of independent clones of mutant mtBALB and wild type control mtB6 murine cybrid cells

ABSTRACT: We generated murine fibroblast cybrid cell lines that have identical nuclear genomes and differ only in their mtDNA. We observed increased cellular proliferation and resistance to apoptosis in the mtBALB compared to the mtB6 cybrid cells, phenotypes seen in malignant cells. Based on these observations we investigated whether these phenotypic differences could be caused by a unique spectrum of nuclear gene expression alterations induced by the mtDNA changes. Microarray analysis (Agilent, 44K mouse whole genome chip) was conducted in order to elucidate the expression profile of three independent clones of mtBALB and mtB6 cybrid cells. Two-condition experiment, mtB6 vs. mtBALB cells. Biological replicates: 4 control replicates, 4 mutant replicates.

ORGANISM(S): Mus musculus

SUBMITTER: Jaroslav Janda

PROVIDER: E-GEOD-21855 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Changes in mitochondrial DNA alter expression of nuclear encoded genes associated with tumorigenesis.

Jandova Jana J Janda Jaroslav J Sligh James E JE

Experimental cell research 20120613 17

We previously reported the presence of a mtDNA mutation hotspot in UV-induced premalignant and malignant skin tumors in hairless mice. We have modeled this change (9821insA) in murine cybrid cells and demonstrated that this alteration in mtDNA associated with mtBALB haplotype can alter the biochemical characteristics of cybrids and subsequently can contribute to significant changes in their behavioral capabilities. This study shows that changes in mtDNA can produce differences in expression leve ...[more]

PMID: 22705584

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