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Identification of down stream genes in dehydration treated NCED3 mutants

ABSTRACT: To understand the Abscisic Acid (ABA) signaling in response to dehydration stress, we performed analysis of gene expression using Arabidopsis wild-type plants and the nced3-2 mutant under dehydration stress. The nced3-2 mutant is an Arabidopsis T-DNA tagged knock-out mutant of the NCED3 gene, which has an essential role in dehydration-inducible ABA biosynthesis. Arabidopsis plants were grown in in soil (verdenite 40 mmÎ¦, Verde Co., Ltd., Kanagawa, Japan) in a cell strainer (Falcon, 40 Î¼m; Corning Inc., NY, USA). Plants were grown at 22Â°C for 3 weeks under illumination (40â60 Î¼mol m-2s-1; 16 h light/8 h darkness). Three-week-old plants were exposed to dehydration stress by being denied water for 6, 24, 48, or 72 h.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Kyonoshin Maruyama

PROVIDER: E-MTAB-4640 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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