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Transcriptional analysis of quorum sensing deficient in Yersinia pestis CO92 at 37M-BM-0C

ABSTRACT: Yersinia pestis, the etiological agent of plague, is able to sense cell density by quorum sensing. The function of quorum sensing in Y. pestis is not clear. Here, the process of quorum sensing was investigated by comparing transcript profiles when three quorum-sensing synthase genes are knocked out. Two strains, M-bM-^HM-^Fpgm (pigmentation-negative) mutant R88 as treatment and quorum sensing null strain R115 with mutations (M-bM-^HM-^Fpgm, M-bM-^HM-^FypeIR, M-bM-^HM-^FyspIR, and M-bM-^HM-^FluxS) as control, are used in this analysis. Six independent RNA samples from R115 cultures were paired with six independent RNA samples from R88 cultures for hybridization to six two-color microarrays. A dye-swap design was used to remove the Cy5 and Cy3 dye bias.

ORGANISM(S): Yersinia pestis

SUBMITTER: Chris Minion

PROVIDER: E-GEOD-21911 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Analysis of autoinducer-2 quorum sensing in Yersinia pestis.

Yu Jing J Madsen Melissa L ML Carruthers Michael D MD Phillips Gregory J GJ Kavanaugh Jeffrey S JS Boyd Jeff M JM Horswill Alexander R AR Minion F Chris FC

Infection and immunity 20130819 11

The autoinducer-2 (AI-2) quorum-sensing system has been linked to diverse phenotypes and regulatory changes in pathogenic bacteria. In the present study, we performed a molecular and biochemical characterization of the AI-2 system in Yersinia pestis, the causative agent of plague. In strain CO92, the AI-2 signal is produced in a luxS-dependent manner, reaching maximal levels of 2.5 μM in the late logarithmic growth phase, and both wild-type and pigmentation (pgm) mutant strains made equivalent l ...[more]

PMID: 23959719

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Transcriptional analysis of luxS-null strain in Yersinia pestis CO92 at 37M-BM-0C

Project description:Yersinia pestis, the etiological agent of plague, is able to sense cell density by quorum sensing. The function of quorum sensing in Y. pestis is not clear. Here, the process of autoinducer 2 (AI-2) quorum sensing was investigated by comparing transcript profiles when luxS gene was knocked out. The luxS gene encodes S-ribosylhomocysteinase which can produce DPD, a precursor of AI-2. The strain M-bM-^HM-^Fpgm (pigmentation-negative) mutant R88 was called wild type. The M-bM-^HM-^Fpgm M-bM-^HM-^FluxS mutant was called control. Six independent RNA samples from R88 were paired with six independent RNA samples from M-bM-^HM-^Fpgm M-bM-^HM-^FluxS mutant cultures for hybridization to six two-color microarrays. A dye-swap design was used to remove the Cy5 and Cy3 dye bias.

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Transcription analysis of AI-2 signal add in experiment in Yersinia pestis CO92 at 37M-BM-0C

Project description:Yersinia pestis, the etiological agent of plague, is able to sense cell density by quorum sensing. The function of quorum sensing in Y. pestis is not clear. Here, the process of autoinducer-2 (AI-2) quorum sensing was investigated by comparing transcript profiles when AI-2 quorum-sensing signal is added in. The strain M-bM-^HM-^Fpgm(pigmentation-negative) mutant R88 was used as wild type. The control consisted of cells grown and treated under the same conditions without added signals. Six independent RNA samples from Y. pestis CO92 M-bM-^HM-^Fpgm cultures were paired with six independent RNA samples from AI-2 signal added cultures for hybridization to six two-color microarrays. A dye-swap design was used to remove the Cy5 and Cy3 dye bias.

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Transcriptional analysis of quorum sensing deficient in Yersinia pestis CO92 at 37°C

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Transcriptional analysis of AHLs signals add in experiment inYersinia pestis CO92 at 37Â°C

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Transcriptional analysis of ypeIR mutation in Yersinia pestis at 37Â°C

Project description:The etiologic agent of bubonic plague, Yersinia pestis, senses cell density-dependent chemical signals to synchronize transcription between cells of the population in a process named quorum sensing. Though the closely related enteric pathogen Y. pseudotuberculosis uses quorum sensing system to regulate motility, the role of YpeIR quorum sensing in Y. pestis has been unclear. YpeIR is one of the AHL quorum sensing system in Y. pestis. In this study we performed transcriptional profiling experiments to identify Y. pestis YpeIR quorum sensing regulated functions at 37Â°C. Six independent RNA samples from Y. pestis CO92 Îpgm ÎypeIR cultures were paired with six independent RNA samples from control Y. pestis CO92 R88 cultures for hybridization to six two-color microarrays. For three arrays, the control RNA sample was labeled with Alexa 555 dye and the experimental RNA sample was labeled with Alexa 647 dye; the dyes were reversed for the other three arrays to account for any dye bias.

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