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Transcriptional analysis of quorum sensing deficient in Yersinia pestis CO92 at 37°C

ABSTRACT: Yersinia pestis, the etiological agent of plague, is able to sense cell density by quorum sensing. The function of quorum sensing in Y. pestis is not clear. Here, the process of quorum sensing was investigated by comparing transcript profiles when three quorum-sensing synthase genes are knocked out. Two strains, ∆pgm (pigmentation-negative) mutant R88 as treatment and quorum sensing null strain R115 with mutations (∆pgm, ∆ypeIR, ∆yspIR, and ∆luxS) as control, are used in this analysis.

ORGANISM(S): Yersinia pestis

PROVIDER: GSE21911 | GEO | 2013/09/05

SECONDARY ACCESSION(S): PRJNA129409

REPOSITORIES: GEO

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Transcriptional analysis of quorum sensing null strain in Yersinia pestis CO92 at 30°C

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Transcriptional analysis of AHLs signals add in experiment inYersinia pestis CO92 at 37°C

Project description:Yersinia pestis, the etiological agent of plague, is able to sense cell density by quorum sensing. The function of quorum sensing in Y. pestis is not clear. Here, the process of AHL quorum sensing was investigated by comparing transcript profiles when two AHL quorum-sensing signals are added in. The strain ∆pgm (pigmentation-negative) mutant R88 was called wild type. The two AHLs signals are N-(3-Oxooctanoyl)-L-homoserine lactone and N-Hexanoyl-DL-homoserine lactone.The control consisted of cells grown and treated under the same conditions without added signals.

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Transcriptional analysis of three quorum sensing signals add in experiment inYersinia pestis CO92 at 37°C

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