Unknown,Transcriptomics,Genomics,Proteomics

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Time-of-day-dependent light-induction of gene expression in the chicken pineal gland


ABSTRACT: Light has a strong effect on whole organism physiology, such as the circadian rhythms that are phase delayed and advanced by light given at early and late subjective night, respectively. Despite the importance of the phase-dependent light responses, little is known about the underlying molecular mechanism. We performed a comprehensive analysis of genes induced by light in a phase-dependent manner in the chicken pineal gland, an organ that represents a unique vertebrate clock system harboring intrinsic light sensitivity. Newborn chicks were entrained to 12-h light/12-h dark cycle for 7 days then transferred to constant darkness for a day to be exposed to light for 1 h from CT (circadian time) 6 (representing subjective day), CT14 (early subjective night) or CT22 (late subjective night). Control animals were kept in the dark without light pulse. The pineal glands were isolated at the end of the 1-h light pulse for gene expression analysis by Affymetrix GeneChip. Each condition contains 2 biological samples.

ORGANISM(S): Gallus gallus

SUBMITTER: Yoshitaka Fukada 

PROVIDER: E-GEOD-21915 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Light-dependent and circadian clock-regulated activation of sterol regulatory element-binding protein, X-box-binding protein 1, and heat shock factor pathways.

Hatori Megumi M   Hirota Tsuyoshi T   Iitsuka Michiko M   Kurabayashi Nobuhiro N   Haraguchi Shogo S   Kokame Koichi K   Sato Ryuichiro R   Nakai Akira A   Miyata Toshiyuki T   Tsutsui Kazuyoshi K   Fukada Yoshitaka Y  

Proceedings of the National Academy of Sciences of the United States of America 20110307 12


The circadian clock is phase-delayed or -advanced by light when given at early or late subjective night, respectively. Despite the importance of the time-of-day-dependent phase responses to light, the underlying molecular mechanism is poorly understood. Here, we performed a comprehensive analysis of light-inducible genes in the chicken pineal gland, which consists of light-sensitive clock cells representing a prototype of the clock system. Light stimulated expression of 62 genes and 40 ESTs by >  ...[more]

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