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Transcriptome analysis of normal erythroid progenitors and erythroleukemia cells

ABSTRACT: We compared the transcriptomes of ES cell derived erythroid progentor cells (ES-EP) and murine erythroleukemia (MEL) cells stably transfected with Gata-1 fused to ER. RNA was isolated from duplicate proliferating cultures of MEL and ES-EP using Affymetrix GeneChip Mouse Gene 1.0 ST.

ORGANISM(S): Mus musculus

SUBMITTER: Sandeep Wontakal

PROVIDER: E-GEOD-21949 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A large gene network in immature erythroid cells is controlled by the myeloid and B cell transcriptional regulator PU.1.

Wontakal Sandeep N SN Guo Xingyi X Will Britta B Shi Minyi M Raha Debasish D Mahajan Milind C MC Weissman Sherman S Snyder Michael M Steidl Ulrich U Zheng Deyou D Skoultchi Arthur I AI

PLoS genetics 20110609 6

PU.1 is a hematopoietic transcription factor that is required for the development of myeloid and B cells. PU.1 is also expressed in erythroid progenitors, where it blocks erythroid differentiation by binding to and inhibiting the main erythroid promoting factor, GATA-1. However, other mechanisms by which PU.1 affects the fate of erythroid progenitors have not been thoroughly explored. Here, we used ChIP-Seq analysis for PU.1 and gene expression profiling in erythroid cells to show that PU.1 regu ...[more]

PMID: 21695229

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